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19 pages, 1401 KiB  
Article
The Identification of a Novel Nucleomodulin MbovP467 of Mycoplasmopsis bovis and Its Potential Contribution in Pathogenesis
by Abdul Raheem, Doukun Lu, Abdul Karim Khalid, Gang Zhao, Yingjie Fu, Yingyu Chen, Xi Chen, Changmin Hu, Jianguo Chen, Huanchun Chen and Aizhen Guo
Cells 2024, 13(7), 604; https://doi.org/10.3390/cells13070604 (registering DOI) - 29 Mar 2024
Abstract
Mycoplasmopsis bovis is a causative agent of crucial diseases in both dairy and beef cattle leading to substantial economic losses. However, limited control measures for M. bovis-related diseases exist due to a lack of understanding about the virulence factors of this pathogen, [...] Read more.
Mycoplasmopsis bovis is a causative agent of crucial diseases in both dairy and beef cattle leading to substantial economic losses. However, limited control measures for M. bovis-related diseases exist due to a lack of understanding about the virulence factors of this pathogen, a common challenge in mycoplasma research. Consequently, this study aimed to characterize a novel nucleomodulin as a virulence-related factor of M. bovis. Employing bioinformatic tools, we initially predicted MbovP467 to be a secreted protein with a nuclear localization signal based on SignalP scores and the cNLS (Nuclear Localization Signal) Mapper, respectively. Subsequently, the MbovP467 gene was synthesized and cloned into a pEGFP plasmid with EGFP labeling to obtain a recombinant plasmid (rpEGFP-MbovP467) and then was also cloned in pET-30a with a consideration for an Escherichia coli codon bias and expressed and purified for the production of polyclonal antibodies against the recombinant MbovP467 protein. Confocal microscopy and a Western blotting assay confirmed the nuclear location of MbovP467 in bovine macrophages (BoMacs). RNA-seq data revealed 220 up-regulated and 20 down-regulated genes in the rpEGFP-MbovP467-treated BoMac group compared to the control group (pEGFP). A GO- and KEGG-enrichment analysis identified associations with inflammatory responses, G protein-coupled receptor signaling pathways, nuclear receptor activity, sequence-specific DNA binding, the regulation of cell proliferation, IL-8, apoptotic processes, cell growth and death, the TNF signaling pathway, the NF-κB signaling pathway, pathways in cancer, and protein families of signaling and cellular processes among the differentially expressed up-regulated mRNAs. Further experiments, investigating cell viability and the inflammatory response, demonstrated that MbovP467 reduces BoMac cell viability and induces the mRNA expression of IL-1β, IL-6, IL-8, TNF-α, and apoptosis in BoMac cells. Further, MbovP467 increased the promoter activity of TNF-α. In conclusion, this study identified a new nucleomodulin, MbovP467, for M. bovis, which might have an important role in M. bovis pathogenesis. Full article
(This article belongs to the Special Issue Recent Advancements in the Regulated Cell Death)
54 pages, 3387 KiB  
Review
Chemical Constituents and Biological Activities of Bruguiera Genus and Its Endophytes: A Review
by Xiongming Luo, Xiaohong Chen, Lingli Zhang, Bin Liu, Lian Xie, Yan Ma, Min Zhang and Xiaobao Jin
Mar. Drugs 2024, 22(4), 158; https://doi.org/10.3390/md22040158 (registering DOI) - 29 Mar 2024
Abstract
The genus Bruguiera, a member of the Rhizophoraceae family, is predominantly found in coastal areas as a mangrove plant, boasting a rich and diverse community of endophytes. This review systematically compiled approximately 496 compounds derived from both the Bruguiera genus and its [...] Read more.
The genus Bruguiera, a member of the Rhizophoraceae family, is predominantly found in coastal areas as a mangrove plant, boasting a rich and diverse community of endophytes. This review systematically compiled approximately 496 compounds derived from both the Bruguiera genus and its associated endophytes, including 152 terpenoids, 17 steroids, 16 sulfides, 44 alkaloids and peptides, 66 quinones, 68 polyketides, 19 flavonoids, 38 phenylpropanoids, 54 aromatic compounds, and 22 other compounds. Among these, 201 compounds exhibited a spectrum of activities, including cytotoxicity, antimicrobial, antioxidant, anti-inflammatory, antiviral, antidiabetic, insecticidal and mosquito repellent, and enzyme inhibitory properties, etc. These findings provided promising lead compounds for drug discovery. Certain similar or identical compounds were found to be simultaneously present in both Bruguiera plants and their endophytes, and the phenomenon of their interaction relationship was discussed. Full article
(This article belongs to the Special Issue Bio-Active Products from Mangrove Ecosystems 2.0)
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23 pages, 2178 KiB  
Article
MRG-T: Mask-Relation-Guided Transformer for Remote Vision-Based Pedestrian Attribute Recognition in Aerial Imagery
by Shun Zhang, Yupeng Li, Xiao Wu, Zunheng Chu and Lingfei Li
Remote Sens. 2024, 16(7), 1216; https://doi.org/10.3390/rs16071216 (registering DOI) - 29 Mar 2024
Abstract
Nowadays, with the rapid development of consumer Unmanned Aerial Vehicles (UAVs), utilizing UAV platforms for visual surveillance has become very attractive, and a key part of this is remote vision-based pedestrian attribute recognition. Pedestrian Attribute Recognition (PAR) is dedicated to predicting multiple attribute [...] Read more.
Nowadays, with the rapid development of consumer Unmanned Aerial Vehicles (UAVs), utilizing UAV platforms for visual surveillance has become very attractive, and a key part of this is remote vision-based pedestrian attribute recognition. Pedestrian Attribute Recognition (PAR) is dedicated to predicting multiple attribute labels of a single pedestrian image extracted from surveillance videos and aerial imagery, which presents significant challenges in the computer vision community due to factors such as poor imaging quality and substantial pose variations. Despite recent studies demonstrating impressive advancements in utilizing complicated architectures and exploring relations, most of them may fail to fully and systematically consider the inter-region, inter-attribute, and region-attribute mapping relations simultaneously and be stuck in the dilemma of information redundancy, leading to the degradation of recognition accuracy. To address the issues, we construct a novel Mask-Relation-Guided Transformer (MRG-T) framework that consists of three relation modeling modules to fully exploit spatial and semantic relations in the model learning process. Specifically, we first propose a Masked Region Relation Module (MRRM) to focus on precise spatial attention regions to extract more robust features with masked random patch training. To explore the semantic association of attributes, we further present a Masked Attribute Relation Module (MARM) to extract intrinsic and semantic inter-attribute relations with an attribute label masking strategy. Based on the cross-attention mechanism, we finally design a Region and Attribute Mapping Module (RAMM) to learn the cross-modal alignment between spatial regions and semantic attributes. We conduct comprehensive experiments on three public benchmarks such as PETA, PA-100K, and RAPv1, and conduct inference on a large-scale airborne person dataset named PRAI-1581. The extensive experimental results demonstrate the superior performance of our method compared to state-of-the-art approaches and validate the effectiveness of mask-relation-guided modeling in the remote vision-based PAR task. Full article
(This article belongs to the Special Issue Signal Processing Theory and Methods in Remote Sensing)
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23 pages, 759 KiB  
Review
Potential New Target for Dry Eye Disease—Oxidative Stress
by Jinghua Bu, Yanbo Liu, Rongrong Zhang, Sijie Lin, Jingbin Zhuang, Le Sun, Lingyu Zhang, Hui He, Rongrong Zong, Yang Wu and Wei Li
Antioxidants 2024, 13(4), 422; https://doi.org/10.3390/antiox13040422 (registering DOI) - 29 Mar 2024
Abstract
Dry eye disease (DED) is a multifactorial condition affecting the ocular surface. It is characterized by loss of tear film homeostasis and accompanied by ocular symptoms that may potentially result in damage to the ocular surface and even vision loss. Unmodifiable risk factors [...] Read more.
Dry eye disease (DED) is a multifactorial condition affecting the ocular surface. It is characterized by loss of tear film homeostasis and accompanied by ocular symptoms that may potentially result in damage to the ocular surface and even vision loss. Unmodifiable risk factors for DED mainly include aging, hormonal changes, and lifestyle issues such as reduced sleep duration, increased screen exposure, smoking, and ethanol consumption. As its prevalence continues to rise, DED has garnered considerable attention, prompting the exploration of potential new therapeutic targets. Recent studies have found that when the production of ROS exceeds the capacity of the antioxidant defense system on the ocular surface, oxidative stress ensues, leading to cellular apoptosis and further oxidative damage. These events can exacerbate inflammation and cellular stress responses, further increasing ROS levels and promoting a vicious cycle of oxidative stress in DED. Therefore, given the central role of reactive oxygen species in the vicious cycle of inflammation in DED, strategies involving antioxidants have emerged as a novel approach for its treatment. This review aims to enhance our understanding of the intricate relationship between oxidative stress and DED, thereby providing directions to explore innovative therapeutic approaches for this complex ocular disorder. Full article
(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)
16 pages, 761 KiB  
Review
The Role of Cytokines in the Pathogenesis and Treatment of Alcoholic Liver Disease
by Giuseppe Guido Maria Scarlata, Carmen Colaci, Marialaura Scarcella, Marcello Dallio, Alessandro Federico, Luigi Boccuto and Ludovico Abenavoli
Diseases 2024, 12(4), 69; https://doi.org/10.3390/diseases12040069 (registering DOI) - 29 Mar 2024
Abstract
Alcoholic liver disease (ALD) is a major cause of chronic liver disease. This term covers a broad spectrum of liver lesions, from simple steatosis to alcoholic hepatitis and cirrhosis. The pathogenesis of ALD is multifactorial and not fully elucidated due to complex mechanisms [...] Read more.
Alcoholic liver disease (ALD) is a major cause of chronic liver disease. This term covers a broad spectrum of liver lesions, from simple steatosis to alcoholic hepatitis and cirrhosis. The pathogenesis of ALD is multifactorial and not fully elucidated due to complex mechanisms related to direct ethanol toxicity with subsequent hepatic and systemic inflammation. The accumulation of pro-inflammatory cytokines and the reduction of anti-inflammatory cytokines promote the development and progression of ALD. To date, there are no targeted therapies to counter the progression of chronic alcohol-related liver disease and prevent acute liver failure. Corticosteroids reduce mortality by acting on the hepatic-systemic inflammation. On the other hand, several studies analyzed the effect of inhibiting pro-inflammatory cytokines and stimulating anti-inflammatory cytokines as potential therapeutic targets in ALD. This narrative review aims to clarify the role of the main cytokines involved in the pathogenesis and treatment of ALD. Full article
(This article belongs to the Section Gastroenterology)
20 pages, 462 KiB  
Article
Advancing User Privacy in Virtual Power Plants: A Novel Zero-Knowledge Proof-Based Distributed Attribute Encryption Approach
by Ruxia Yang, Hongchao Gao, Fangyuan Si and Jun Wang
Electronics 2024, 13(7), 1283; https://doi.org/10.3390/electronics13071283 (registering DOI) - 29 Mar 2024
Abstract
In virtual power plants, diverse business scenarios involving user data, such as queries, transactions, and sharing, pose significant privacy risks. Traditional attribute-based encryption (ABE) methods, while supporting fine-grained access, fall short of fully protecting user privacy as they require attribute input, leading to [...] Read more.
In virtual power plants, diverse business scenarios involving user data, such as queries, transactions, and sharing, pose significant privacy risks. Traditional attribute-based encryption (ABE) methods, while supporting fine-grained access, fall short of fully protecting user privacy as they require attribute input, leading to potential data leaks. Addressing these limitations, our research introduces a novel privacy protection scheme using zero-knowledge proof and distributed attribute-based encryption (DABE). This method innovatively employs Merkel trees for aggregating user attributes and constructing commitments for zero-knowledge proof verification, ensuring that user attributes and access policies remain confidential. Our solution not only enhances privacy but also fortifies security against man-in-the-middle and replay attacks, offering attribute indistinguishability and tamper resistance. A comparative performance analysis demonstrates that our approach outperforms existing methods in efficiency, reducing time, cost, and space requirements. These advancements mark a significant step forward in ensuring robust user privacy and data security in virtual power plants. Full article
(This article belongs to the Special Issue Knowledge Information Extraction Research)
15 pages, 1637 KiB  
Article
Wearable Movement Exploration Device with Machine Learning Algorithm for Screening and Tracking Diabetic Neuropathy—A Cross-Sectional, Diagnostic, Comparative Study
by Goran Radunovic, Zoran Velickovic, Slavica Pavlov-Dolijanovic, Sasa Janjic, Biljana Stojic, Irena Jeftovic Velkova, Nikola Suljagic and Ivan Soldatovic
Biosensors 2024, 14(4), 166; https://doi.org/10.3390/bios14040166 (registering DOI) - 29 Mar 2024
Abstract
Background: Diabetic neuropathy is one of the most common complications of diabetes mellitus. The aim of this study is to evaluate the Moveo device, a novel device that uses a machine learning (ML) algorithm to detect and track diabetic neuropathy. The Moveo device [...] Read more.
Background: Diabetic neuropathy is one of the most common complications of diabetes mellitus. The aim of this study is to evaluate the Moveo device, a novel device that uses a machine learning (ML) algorithm to detect and track diabetic neuropathy. The Moveo device comprises 4 sensors positioned on the back of the hands and feet accompanied by a mobile application that gathers data and ML algorithms that are hosted on a cloud platform. The sensors measure movement signals, which are then transferred to the cloud through the mobile application. The cloud triggers a pipeline for feature extraction and subsequently feeds the ML model with these extracted features. Methods: The pilot study included 23 participants. Eleven patients with diabetes and suspected diabetic neuropathy were included in the experimental group. In the control group, 8 patients had suspected radiculopathy, and 4 participants were healthy. All participants underwent an electrodiagnostic examination (EDx) and a Moveo examination, which consists of sensors placed on the feet and back of the participant’s hands and use of the mobile application. The participant performs six tests that are part of a standard neurological examination, and a ML algorithm calculates the probability of diabetic neuropathy. A user experience questionnaire was used to compare participant experiences with regard to both methods. Results: The total accuracy of the algorithm is 82.1%, with 78% sensitivity and 87% specificity. A high linear correlation up to 0.722 was observed between Moveo and EDx features, which underpins the model’s adequacy. The user experience questionnaire revealed that the majority of patients preferred the less painful method. Conclusions: Moveo represents an accurate, easy-to-use device suitable for home environments, showing promising results and potential for future usage. Full article
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16 pages, 2132 KiB  
Article
Force Production and Electromyographic Activity during Different Flywheel Deadlift Exercises
by Sergio Maroto-Izquierdo, David García-López, Marco Beato, Iker J. Bautista, José L. Hernández-Davó, Javier Raya-González and Fernando Martín-Rivera
Sports 2024, 12(4), 95; https://doi.org/10.3390/sports12040095 (registering DOI) - 29 Mar 2024
Abstract
This study aimed to characterize and compare force production and muscle activity during four flywheel deadlift exercises (bilateral [Bi] vs. unilateral [Uni]) with different loading conditions (vertical [Ver] vs. horizontal [Hor]). Twenty-three team-sport athletes underwent assessments for exercise kinetics (hand-grip force), along with [...] Read more.
This study aimed to characterize and compare force production and muscle activity during four flywheel deadlift exercises (bilateral [Bi] vs. unilateral [Uni]) with different loading conditions (vertical [Ver] vs. horizontal [Hor]). Twenty-three team-sport athletes underwent assessments for exercise kinetics (hand-grip force), along with surface electromyography (sEMG) of the proximal (BFProx) and medial biceps femoris (BFMed), semitendinosus (ST), and gluteus medius (GM). Mean and peak force were highest (p < 0.001) in Bi + Ver compared with Bi + Hor, Uni + Ver, and Uni + Hor. Although no significant differences were observed between Bi + Hor and Uni + Ver, both variants showed higher (p < 0.001) average force and peak eccentric force when compared with Uni + Hor. The presence of eccentric overload was only observed in the vertically loaded variants. Bi + Ver and Uni + Ver showed higher (p < 0.05) sEMG levels in BFProx and BFMed compared with the Uni + Hor variant. In addition, Uni + Ver registered the largest GM and ST sEMG values. In conclusion, the vertical variants of the flywheel deadlift exercise led to higher muscle force production and sEMG compared with their horizontal counterparts. Both Bi + Ver and Uni + Ver may be effective in promoting an increase in hamstring muscles activity and muscle force at long muscle length, while the Uni + Ver variant may be more effective in promoting GM and ST involvement. Full article
17 pages, 2079 KiB  
Article
Characteristics of Soil Particle Sizes and Fractal Parameters under Different Plantation Types of Populus alba
by Haonian Li, Zhongju Meng, Xiaomen Ren and Xiaoyan Chen
Forests 2024, 15(4), 632; https://doi.org/10.3390/f15040632 (registering DOI) - 29 Mar 2024
Abstract
Vegetation plays a leading role in restoring desert ecosystems and increasing productivity. In this study, we elucidate the improvement effects of different restoration areas of Populus alba on the soil particle distribution, sedimentation environment, and fractal characteristics. We selected the restoration areas of [...] Read more.
Vegetation plays a leading role in restoring desert ecosystems and increasing productivity. In this study, we elucidate the improvement effects of different restoration areas of Populus alba on the soil particle distribution, sedimentation environment, and fractal characteristics. We selected the restoration areas of P. alba × Caragana korshinskii (YN), P. alba × Hedysarum leave (YY), and P. alba × Hedysarum scoparium (YH), which have a history of twenty-one years. We analyzed the soil nutrients, soil particle size, soil particle size parameters, soil fractal dimension (D) values, and soil multifractal parameters at soil depths of 0–80 cm. We found that the YN, YY, and YH significantly increased the soil nutrients and soil fine particles (p < 0.05) and changed the deposition pattern of the soil particles in the sandy area. The YN, YY, and YH promote soil particle refinement and reduce the sorting performance of the soil particles. The vegetation promotes extremely positive-skewed and very leptokurtic soil particle distributions. The D values in the YN, YY, and YH restoration areas increased by 7.62%–27.94%, 7.36%–26.28%, and 7.10%–17.92%, respectively, relative to those of the LS. The construction of the different restoration areas of P. alba has made the distribution of the soil particles nonuniform. Compared with the YY and YH plantations, the distribution range of the soil particles in the YN plantation is wider, and the distribution heterogeneity is greater. In addition, we found that the fractal parameters are influenced by the soil physicochemical properties, the depositional environment, and vegetation factors. Therefore, we believe that D values and multifractal parameters are necessary as additional information for desert soil texture improvement. The results of this study provide a scientific and theoretical basis for the future revegetation of deserts. Full article
(This article belongs to the Section Forest Soil)
8 pages, 228 KiB  
Perspective
Sustainable-by-Design Approach of Active Catalysts to Produce Reactive Oxygen Species in Water Matrices
by Prisco Prete
Sustain. Chem. 2024, 5(2), 60-67; https://doi.org/10.3390/suschem5020005 (registering DOI) - 29 Mar 2024
Abstract
An overview of the latest advances in the design of active catalysts with the ability to promote (photo) Fenton processes in water from a Green Chemistry perspective is discussed herein. A critical evaluation of the most relevant advances has been disclosed, and a [...] Read more.
An overview of the latest advances in the design of active catalysts with the ability to promote (photo) Fenton processes in water from a Green Chemistry perspective is discussed herein. A critical evaluation of the most relevant advances has been disclosed, and a brief perspective is presented about what is needed to fill the gap of knowledge in this field. Full article
12 pages, 1102 KiB  
Article
PRESERFLO™ Microshunt: 1-Year Results of a 25-Gauge vs. 27-Gauge Needle Tract
by Stefan Steiner, Hemma Resch, Barbara Kiss and Clemens Vass
J. Clin. Med. 2024, 13(7), 1979; https://doi.org/10.3390/jcm13071979 (registering DOI) - 29 Mar 2024
Abstract
Background: The purpose of this study was to evaluate the effectiveness and safety of the PreserFlo™ microshunt (PMS) using a 25-Gauge vs. 27-Gauge needle tract. Methods: This is a prospective postoperative examination of 60 glaucoma eyes that received a PMS. The main [...] Read more.
Background: The purpose of this study was to evaluate the effectiveness and safety of the PreserFlo™ microshunt (PMS) using a 25-Gauge vs. 27-Gauge needle tract. Methods: This is a prospective postoperative examination of 60 glaucoma eyes that received a PMS. The main outcome measures were intraocular pressure (IOP), glaucoma drug score (GDS), Kaplan–Meier success rates, complications, and secondary intervention rates. Two subgroups were formed for data comparison: 27-Gauge (27G), and 25-Gauge (25G). Success was defined as IOP < 18 mmHg together with ≥20% IOP reduction with medication allowed (qualified success = QS18) or not (full success = FS18). Results: IOP and GDS were reduced from baseline to the 1-year study visit as follows: All eyes from 23.4 ± 8.6 mmHg (3.1 ± 0.9) to 15.1 ± 5.9 mmHg (0.8 ± 1.1); 25G from 24.2 ± 7.3 mmHg (3.0 ± 0.8) to 12.7 ± 2.7 mmHg (0.5 ± 0.8); and 27G from 23.1 ± 9.2 mmHg (3.1 ± 1.0) to 16.2 ± 6.7 mmHg (0.9 ± 1.2). IOP at one year was lower in the 25G group compared to the 27G group (p = 0.035). Bleb needling was required in eight (13.3%) eyes and open bleb revisions in three (5.0%). Transient hypotony occurred in 21% and choroidal effusion in 8% of all eyes. Choroidal effusions were more frequent in the 25G group (21%) compared to the 27G group (2%, p = 0.031). One-year success rates were significantly higher in the 25G group compared to the 27G group for both QS18 (25G: 67.9% vs. 27G: 35.7%, p = 0.002) and FS18 (25G: 63.6% vs. 27G: 29.2%, p = 0.007). Conclusions: The PreserFlo microshunt is an effective and safe glaucoma surgery with a low rate of bleb revisions or needlings. We show that the 25G needle tract might be more efficient for IOP control at the cost of increased IOP-related complications compared to 27G. Full article
(This article belongs to the Section Ophthalmology)
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17 pages, 1335 KiB  
Review
Alterations in Proteostasis Mechanisms in Niemann–Pick Type C Disease
by Iris Valeria Servín Mu?oz, Daniel Ortu?o-Sahagún, Christian Gri?án-Ferré, Mercè Pallàs and Celia González-Castillo
Int. J. Mol. Sci. 2024, 25(7), 3806; https://doi.org/10.3390/ijms25073806 (registering DOI) - 29 Mar 2024
Abstract
Niemann–Pick Type C (NPC) represents an autosomal recessive disorder with an incidence rate of 1 in 150,000 live births, classified within lysosomal storage diseases (LSDs). The abnormal accumulation of unesterified cholesterol characterizes the pathophysiology of NPC. This phenomenon is not unique to NPC, [...] Read more.
Niemann–Pick Type C (NPC) represents an autosomal recessive disorder with an incidence rate of 1 in 150,000 live births, classified within lysosomal storage diseases (LSDs). The abnormal accumulation of unesterified cholesterol characterizes the pathophysiology of NPC. This phenomenon is not unique to NPC, as analogous accumulations have also been observed in Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative disorders. Interestingly, disturbances in the folding of the mutant protein NPC1 I1061T are accompanied by the aggregation of proteins such as hyperphosphorylated tau, α-synuclein, TDP-43, and β-amyloid peptide. These accumulations suggest potential disruptions in proteostasis, a regulatory process encompassing four principal mechanisms: synthesis, folding, maintenance of folding, and protein degradation. The dysregulation of these processes leads to excessive accumulation of abnormal proteins that impair cell function and trigger cytotoxicity. This comprehensive review delineates reported alterations across proteostasis mechanisms in NPC, encompassing changes in processes from synthesis to degradation. Additionally, it discusses therapeutic interventions targeting pharmacological facets of proteostasis in NPC. Noteworthy among these interventions is valproic acid, a histone deacetylase inhibitor (HDACi) that modulates acetylation during NPC1 synthesis. In addition, various therapeutic options addressing protein folding modulation, such as abiraterone acetate, DHBP, calnexin, and arimoclomol, are examined. Additionally, treatments impeding NPC1 degradation, exemplified by bortezomib and MG132, are explored as potential strategies. This review consolidates current knowledge on proteostasis dysregulation in NPC and underscores the therapeutic landscape targeting diverse facets of this intricate process. Full article
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3 pages, 158 KiB  
Editorial
Advanced Polarimetry and Polarimetric Imaging
by Xiaobo Li, Fei Liu and Jian Liang
Photonics 2024, 11(4), 317; https://doi.org/10.3390/photonics11040317 (registering DOI) - 29 Mar 2024
Abstract
Polarization, a core attribute of light waves, offers insights into light’s physical properties and its interactions with materials [...] Full article
(This article belongs to the Special Issue Advanced Polarimetry and Polarimetric Imaging)
12 pages, 1329 KiB  
Article
Enhancing the Fermentation Process in Biogas Production from Animal and Plant Waste Substrates in the Southeastern Region of Bulgaria
by Angel Terziev, Penka Zlateva and Martin Ivanov
Fermentation 2024, 10(4), 187; https://doi.org/10.3390/fermentation10040187 (registering DOI) - 29 Mar 2024
Abstract
Annually, a huge amount of waste from plant biomass and animal manure is produced from agriculture and animal farming. Many studies provide information on the biomethane potential of agricultural and livestock wastes, but only a few studies have investigated the application of the [...] Read more.
Annually, a huge amount of waste from plant biomass and animal manure is produced from agriculture and animal farming. Many studies provide information on the biomethane potential of agricultural and livestock wastes, but only a few studies have investigated the application of the substrates in combination. The objective of the study is to enhance the fermentation process in the digester for biogas production, obtained from animal and plant waste substrates. In four batch processes for three months, the temperatures and the residence time of the substrates in the fermenter were analyzed. Simultaneously, electricity and thermal energy were produced via cogeneration units, which were exported to the public grid and city heating network. The plant substrate is a silage mixture of corn and wheat waste. The animal substrate is a mixture of beef and pig manure. Animal and vegetable waste raw materials are collected and transported to the site, located in the region of southeastern Bulgaria. The total annual consumption of animal and plant waste is 17,971 t/year. The enhancement of the process leads to the production of 1,506,000 Nm3 CH4/a of methane, the generation of which requires 299.63 MWh/a of electricity and 649.09 MWh/a thermal energy. Full article
(This article belongs to the Special Issue Biofuels Production and Processing Technology: 3rd Edition)
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15 pages, 2078 KiB  
Article
Synergistic Antibacterial Effects of Gallate Containing Compounds with Silver Nanoparticles in Gallate Crossed Linked PVA Hydrogel Films
by John Jackson and Claudia Helena Dietrich
Antibiotics 2024, 13(4), 312; https://doi.org/10.3390/antibiotics13040312 (registering DOI) - 29 Mar 2024
Abstract
Currently available silver-based antiseptic wound dressings have limited patient effectiveness. There exists a need for wound dressings that behave as comfortable degradable hydrogels with a strong antibiotic potential. The objectives of this project were to investigate the combined use of gallates (either epi [...] Read more.
Currently available silver-based antiseptic wound dressings have limited patient effectiveness. There exists a need for wound dressings that behave as comfortable degradable hydrogels with a strong antibiotic potential. The objectives of this project were to investigate the combined use of gallates (either epi gallo catechin gallate (EGCG), Tannic acid, or Quercetin) as both PVA crosslinking agents and as potential synergistic antibiotics in combination with silver nanoparticles. Crosslinking was assessed gravimetrically, silver and gallate release was measured using inductively coupled plasma and HPLC methods, respectively. Synergy was measured using 96-well plate FICI methods and in-gel antibacterial effects were measured using planktonic CFU assays. All gallates crosslinked PVA with optimal extended swelling obtained using EGCG or Quercetin at 14% loadings (100 mg in 500 mg PVA with glycerol). All three gallates were synergistic in combination with silver nanoparticles against both gram-positive and -negative bacteria. In PVA hydrogel films, silver nanoparticles with EGCG or Quercetin more effectively inhibited bacterial growth in CFU counts over 24 h as compared to films containing single agents. These biocompatible natural-product antibiotics, EGCG or Quercetin, may play a dual role of providing stable PVA hydrogel films and a powerful synergistic antibiotic effect in combination with silver nanoparticles. Full article
(This article belongs to the Special Issue Nanocarriers-Based Antimicrobial Drug Delivery)
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12 pages, 1113 KiB  
Article
Genome-Wide Association Analysis Uncovers Genes Associated with Resistance to Head Smut Pathotype 5 in Senegalese Sorghum Accessions
by Ezekiel Ahn, Louis K. Prom, Sunchung Park, Zhenbin Hu and Clint W. Magill
Plants 2024, 13(7), 977; https://doi.org/10.3390/plants13070977 (registering DOI) - 29 Mar 2024
Abstract
A newly documented pathotype 5 of the soil-borne fungus Sporisorium reilianum, causing head smut in sorghum, was tested against 153 unexplored Senegalese sorghum accessions. Among the 153 sorghum accessions tested, 63 (41%) exhibited complete resistance, showing no signs of infection by the [...] Read more.
A newly documented pathotype 5 of the soil-borne fungus Sporisorium reilianum, causing head smut in sorghum, was tested against 153 unexplored Senegalese sorghum accessions. Among the 153 sorghum accessions tested, 63 (41%) exhibited complete resistance, showing no signs of infection by the fungus. The remaining 90 accessions (59%) displayed varying degrees of susceptibility. Sorghum responses against S. reilianum were explored to analyze the potential link with previously known seed morphology-related traits and new phenotype data from 59 lines for seed weight. A genome-wide association study (GWAS) screened 297,876 SNPs and identified highly significant associations (p < 1 × 10−5) with head smut resistance in sorghum. By mapping these significant SNPs to the reference genome, this study revealed 35 novel candidate defense genes potentially involved in disease resistance. Full article
(This article belongs to the Special Issue Molecular Biology and Genomics of Plant-Pathogen Interactions)
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16 pages, 4537 KiB  
Article
Variations in Essential Oil Biological Activities of Female Cones at Different Developmental Stages from Azorean Cryptomeria japonica (Thunb. ex L.f.) D. Don (Cupressaceae)
by Alexandre Janeiro, Ana Lima, Filipe Arruda, Tanner Wortham, T?nia Rodrigues, José Baptista and Elisabete Lima
Separations 2024, 11(4), 102; https://doi.org/10.3390/separations11040102 (registering DOI) - 29 Mar 2024
Abstract
In the Azores Archipelago, Cryptomeria japonica is, currently, the most cultivated forestry tree for timber production, landscaping, and gardening, generating large amounts of foliage waste that is used for local essential oils (EOs) production. However, the existing literature on the biological potential of [...] Read more.
In the Azores Archipelago, Cryptomeria japonica is, currently, the most cultivated forestry tree for timber production, landscaping, and gardening, generating large amounts of foliage waste that is used for local essential oils (EOs) production. However, the existing literature on the biological potential of EOs from different C. japonica foliage parts, such as female cones (FC), remains limited. Thus, in the present study, EOs extracted by hydrodistillation from Azorean C. japonica immature and mature FC (IFC and MFC), as well as some major EO components, were screened for their: (i) antioxidant capacity, evaluated by DPPH free-radical-scavenging activity (FRSA) and β-carotene-linoleic acid bleaching activity (BCBA), (ii) antimicrobial activities, assessed by the disc diffusion method against eight bacteria and one fungus, and (iii) toxicity against Artemia salina. Among both FC EO samples, the IFC EO exhibited the best DPPH–FRSA, BCBA, and growth inhibitory activity against Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, and Penicillium italicum, as well as a slightly increased toxic potential, due to their differential compositions, as assessed by GC–MS analysis. Thus, the FC’s maturation process decreased their EOs’ bioactivities. In conclusion, this finding could help in determining the optimal developmental stage for enhancing the antioxidant and antimicrobial compounds content in FC EOs. In turn, this contributes to increasing the commercial potential of C. japonica’s EO industry. Full article
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16 pages, 3107 KiB  
Review
The Interplay between Glioblastoma Cells and Tumor Microenvironment: New Perspectives for Early Diagnosis and Targeted Cancer Therapy
by Assunta Virtuoso, Giuseppa D’Amico, Federica Scalia, Ciro De Luca, Michele Papa, Grazia Maugeri, Velia D’Agata, Celeste Caruso Bavisotto and Agata Grazia D’Amico
Brain Sci. 2024, 14(4), 331; https://doi.org/10.3390/brainsci14040331 (registering DOI) - 29 Mar 2024
Abstract
Glioblastoma multiforme (GBM) stands out as the most tremendous brain tumor, constituting 60% of primary brain cancers, accompanied by dismal survival rates. Despite advancements in research, therapeutic options remain limited to chemotherapy and surgery. GBM molecular heterogeneity, the intricate interaction with the tumor [...] Read more.
Glioblastoma multiforme (GBM) stands out as the most tremendous brain tumor, constituting 60% of primary brain cancers, accompanied by dismal survival rates. Despite advancements in research, therapeutic options remain limited to chemotherapy and surgery. GBM molecular heterogeneity, the intricate interaction with the tumor microenvironment (TME), and non-selective treatments contribute to the neoplastic relapse. Diagnostic challenges arise from GBM advanced-stage detection, necessitating the exploration of novel biomarkers for early diagnosis. Using data from the literature and a bioinformatic tool, the current manuscript delineates the molecular interplay between human GBM, astrocytes, and myeloid cells, underscoring selected protein pathways belonging to astroglia and myeloid lineage, which can be considered for targeted therapies. Moreover, the pivotal role of extracellular vesicles (EVs) in orchestrating a favorable microenvironment for cancer progression is highlighted, suggesting their utility in identifying biomarkers for GBM early diagnosis. Full article
(This article belongs to the Section Molecular and Cellular Neuroscience)
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13 pages, 3895 KiB  
Article
Advancing Sustainable Marine Exploration: Highly Efficient Photonic Radar for Underwater Navigation Systems under the Impact of Different Salinity Levels
by Aras Aldawoodi and Hasan ?akir Bilge
Sustainability 2024, 16(7), 2851; https://doi.org/10.3390/su16072851 (registering DOI) - 29 Mar 2024
Abstract
The exploration of underwater environments for applications like environmental monitoring, scientific research, and surveillance has propelled the significance of underwater wireless navigation. Light waves have emerged as a promising solution, offering the potential to achieve the required data rates and propagation speeds. However, [...] Read more.
The exploration of underwater environments for applications like environmental monitoring, scientific research, and surveillance has propelled the significance of underwater wireless navigation. Light waves have emerged as a promising solution, offering the potential to achieve the required data rates and propagation speeds. However, underwater optical wireless navigation faces challenges, particularly limited range. This research investigates a novel FMCW (frequency-modulated continuous wave)-based photonic radar system’s efficacy in detecting underwater vehicles across diverse salinity levels and distances. Numerical simulation evaluations reveal distinct signal-to-noise ratios (SNR) and detected power peaks corresponding to varying salinity levels, demonstrating the system’s sensitivity. At 5 g/L salinity, the detected power peaked at −95 dBm, decreasing to −105 dBm at 15 g/L. SNR analysis indicates robust detection within a 4 m range, with challenges emerging at extended ranges and higher salinity. Despite these challenges, the system shows promise for near-range underwater navigation, contributing to sustainable marine exploration by enhancing the accuracy and efficiency of underwater monitoring systems. This advancement aligns with the goals of sustainable development by supporting the protection of marine ecosystems, promoting scientific understanding of underwater environments, and aiding in the sustainable management of marine resources. Full article
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16 pages, 6324 KiB  
Article
Simultaneous High-Speed Video Laryngoscopy and Acoustic Aerodynamic Recordings during Vocal Onset of Variable Sound Pressure Level: A Preliminary Study
by Peak Woo
Bioengineering 2024, 11(4), 334; https://doi.org/10.3390/bioengineering11040334 (registering DOI) - 29 Mar 2024
Abstract
Voicing: requires frequent starts and stops at various sound pressure levels (SPL) and frequencies. Prior investigations using rigid laryngoscopy with oral endoscopy have shown variations in the duration of the vibration delay between normal and abnormal subjects. However, these studies were not physiological [...] Read more.
Voicing: requires frequent starts and stops at various sound pressure levels (SPL) and frequencies. Prior investigations using rigid laryngoscopy with oral endoscopy have shown variations in the duration of the vibration delay between normal and abnormal subjects. However, these studies were not physiological because the larynx was viewed using rigid endoscopes. We adapted a method to perform to perform simultaneous high-speed naso-endoscopic video while simultaneously acquiring the sound pressure, fundamental frequency, airflow rate, and subglottic pressure. This study aimed to investigate voice onset patterns in normophonic males and females during the onset of variable SPL and correlate them with acoustic and aerodynamic data. Materials and Methods: Three healthy males and three healthy females were studied by simultaneous high-speed video laryngoscopy and recording with the production of the gesture [pa:pa:] at soft, medium, and loud voices. The fiber optic endoscope was threaded through a pneumotachograph mask for the simultaneous recording and analysis of acoustic and aerodynamic data. Results: The average increase in the sound pressure level (SPL) for the group was 15 dB, from 70 to 85 dB. The fundamental frequency increased by an average of 10 Hz. The flow was increased in two subjects, reduced in two subjects, and remained the same in two subjects as the SPL increased. There was a steady increase in the subglottic pressure from soft to loud phonation. Compared to soft to medium phonation, a significant increase in glottal resistance was observed with medium-to-loud phonation. Videokymogram analysis showed the onset of vibration for all voiced tokens without the need for full glottis closure. In loud phonation, there is a more rapid onset of a larger amplitude and prolonged closure of the glottal cycle; however, more cycles are required to achieve the intended SPL. There was a prolonged closed phase during loud phonation. Fast Fourier transform (FFT) analysis of the kymography waveform signal showed a more significant second- and third-harmonic energy above the fundamental frequency with loud phonation. There was an increase in the adjustments in the pharynx with the base of the tongue tilting, shortening of the vocal folds, and pharyngeal constriction. Conclusion: Voice onset occurs in all modalities, without the need for full glottal closure. There was a more significant increase in glottal resistance with loud phonation than that with soft or middle phonation. Vibration analysis of the voice onset showed that more time was required during loud phonation before the oscillation stabilized to a steady state. With increasing SPL, there were significant variations in vocal tract adjustments. The most apparent change was the increase in tongue tension with posterior displacement of the epiglottis. There was an increase in pre-phonation time during loud phonation. Patterns of muscle tension dysphonia with laryngeal squeezing, shortening of the vocal folds, and epiglottis tilting with increasing loudness are features of loud phonation. These observations show that flexible high-speed video laryngoscopy can reveal observations that cannot be observed with rigid video laryngoscopy. An objective analysis of the digital kymography signal can be conducted in selected cases. Full article
(This article belongs to the Special Issue The Biophysics of Vocal Onset)
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12 pages, 788 KiB  
Article
Myelodysplastic Neoplasms (MDS) with Ring Sideroblasts or SF3B1 Mutations: The Improved Clinical Utility of World Health Organization and International Consensus Classification 2022 Definitions, a Single-Centre Retrospective Chart Review
by Shamim Mortuza, Benjamin Chin-Yee, Tyler E. James, Ian H. Chin-Yee, Benjamin D. Hedley, Jenny M. Ho, Lalit Saini, Alejandro Lazo-Langner, Laila Schenkel, Pratibha Bhai, Bekim Sadikovic, Jonathan Keow, Nikhil Sangle and Cyrus C. Hsia
Curr. Oncol. 2024, 31(4), 1762-1773; https://doi.org/10.3390/curroncol31040134 (registering DOI) - 29 Mar 2024
Abstract
Myelodysplastic neoplasms (MDS) with ring sideroblasts (RS) are diagnosed via bone marrow aspiration in the presence of either (i) ≥15% RS or (ii) 5–14% RS and an SF3B1 mutation. In the MEDALIST trial and in an interim analysis of the COMMANDS trial, lower-risk [...] Read more.
Myelodysplastic neoplasms (MDS) with ring sideroblasts (RS) are diagnosed via bone marrow aspiration in the presence of either (i) ≥15% RS or (ii) 5–14% RS and an SF3B1 mutation. In the MEDALIST trial and in an interim analysis of the COMMANDS trial, lower-risk MDS-RS patients had decreased transfusion dependency with luspatercept treatment. A total of 6817 patients with suspected hematologic malignancies underwent molecular testing using a next-generation-sequencing-based genetic assay and 395 MDS patients, seen at our centre from 1 January 2018 to 31 May 2023, were reviewed. Of these, we identified 39 evaluable patients as having lower-risk MDS with SF3B1 mutations: there were 20 (51.3%) males and 19 (48.7%) females, with a median age of 77 years (range of 57 to 92). Nineteen (48.7%) patients had an isolated SF3B1 mutation with a mean variant allele frequency of 35.2% +/− 8.1%, ranging from 7.4% to 46.0%. There were 29 (74.4%) patients with ≥15% RS, 6 (15.4%) with 5 to 14% RS, one (2.6%) with 1% RS, and 3 (7.7%) with no RS. Our study suggests that a quarter of patients would be missed based on the morphologic criterion of only using RS greater than 15% and supports the revised 2022 definitions of the World Health Organization (WHO) and International Consensus Classification (ICC), which shift toward molecularly defined subtypes of MDS and appropriate testing. Full article
(This article belongs to the Special Issue The Molecular Pathology of Myelodysplastic Syndromes)
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14 pages, 2081 KiB  
Systematic Review
Efficacy and Safety of Anti-CD38 Monoclonal Antibodies in Patients with Relapsed or Refractory Multiple Myeloma: A Meta-Analysis of Randomized Clinical Trials
by Francisco Cezar Aquino de Moraes, Vitor Kendi Tsuchiya Sano, Artur de Oliveira Macena L?bo, Francinny Alves Kelly, Victória Morbach, Eric Pasqualotto and Rommel Mario Rodríguez Burbano
J. Pers. Med. 2024, 14(4), 360; https://doi.org/10.3390/jpm14040360 (registering DOI) - 29 Mar 2024
Abstract
The benefit of associating anti-CD38 monoclonal antibodies to proteasome inhibitor (PI)/immunomodulatory agent (IA) and dexamethasone in the treatment of patients with relapsed or refractory multiple myeloma (MM) remains unclear. PubMed, Embase, and Cochrane Library databases were searched for randomized controlled trials that investigated [...] Read more.
The benefit of associating anti-CD38 monoclonal antibodies to proteasome inhibitor (PI)/immunomodulatory agent (IA) and dexamethasone in the treatment of patients with relapsed or refractory multiple myeloma (MM) remains unclear. PubMed, Embase, and Cochrane Library databases were searched for randomized controlled trials that investigated the addition of anti-CD38 monoclonal antibodies to a therapy composed of PI/IA and dexamethasone versus PI/IA and dexamethasone alone for treating relapsed or refractory MM. Hazard ratios (HRs) or risk ratios (RRs) were computed for binary endpoints, with 95% confidence intervals (CIs). Six studies comprising 2191 patients were included. Anti-CD38 monoclonal antibody significantly improved progression-free survival (HR 0.52; 95% CI 0.43–0.61; p < 0.001) and overall survival (HR 0.72; 95% CI 0.63–0.83; p < 0.001). There was a significant increase in hematological adverse events, such as neutropenia (RR 1.41; 95% CI 1.26–1.58; p < 0.01) and thrombocytopenia (RR 1.14; 95% CI 1.02–1.27; p = 0.02), in the group treated with anti-CD38 monoclonal antibody. Also, there was a significant increase in non-hematological adverse events, such as dyspnea (RR 1.72; 95% CI 1.38–2.13; p < 0.01) and pneumonia (RR 1.34; 95% CI 1.13–1.59; p < 0.01), in the group treated with anti-CD38 monoclonal antibody. In conclusion, the incorporation of an anti-CD38 monoclonal antibody demonstrated a promising prospect for reshaping the established MM treatment paradigms. Full article
(This article belongs to the Section Personalized Therapy and Drug Delivery)
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12 pages, 3958 KiB  
Article
Experimental Study on the Adhesion of Abalone to Surfaces with Different Morphologies
by Peng Xi, Yanqi Qiao, Qian Cong and Qingliang Cui
Biomimetics 2024, 9(4), 206; https://doi.org/10.3390/biomimetics9040206 (registering DOI) - 29 Mar 2024
Abstract
To date, research on abalone adhesion has primarily analyzed the organism’s adhesion to smooth surfaces, with few studies on adhesion to non-smooth surfaces. The present study examined the surface morphology of the abalone’s abdominal foot, followed by measuring the adhesive force of the [...] Read more.
To date, research on abalone adhesion has primarily analyzed the organism’s adhesion to smooth surfaces, with few studies on adhesion to non-smooth surfaces. The present study examined the surface morphology of the abalone’s abdominal foot, followed by measuring the adhesive force of the abalone on a smooth force measuring plate and five force measuring plates with different surface morphologies. Next, the adhesion mechanism of the abdominal foot was analyzed. The findings indicated that the abdominal foot of the abalone features numerous stripe-shaped folds on its surface. The adhesion of the abalone to a fine frosted glass plate, a coarse frosted glass plate, and a quadrangular conical glass plate was not significantly different from that on a smooth glass plate. However, the organism’s adhesion to a small lattice pit glass plate and block pattern glass plate was significantly different. The abalone could effectively adhere to the surface of the block pattern glass plate using the elasticity of its abdominal foot during adhesion but experienced difficulty in completely adhering to the surface of the quadrangular conical glass plate. The abdominal foot used its elasticity to form an independent sucker system with each small lattice pit, significantly improving adhesion to the small lattice pit glass plate. The elasticity of the abalone’s abdominal foot created difficulty in handling slight morphological size changes in roughness, resulting in no significant differences in its adhesion to the smooth glass plate. Full article
(This article belongs to the Special Issue Biological Attachment Systems and Biomimetics)
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