Advancing Open Science
for more than 25 years
Supporting academic communities
since 1996
 
39 pages, 754 KiB  
Review
Endovascular Drug Delivery
by Claudiu N. Lungu, Andreea Creteanu and Mihaela C. Mehedinti
Life 2024, 14(4), 451; https://doi.org/10.3390/life14040451 (registering DOI) - 28 Mar 2024
Abstract
Drug-eluting stents (DES) and balloons revolutionize atherosclerosis treatment by targeting hyperplastic tissue responses through effective local drug delivery strategies. This review examines approved and emerging endovascular devices, discussing drug release mechanisms and their impacts on arterial drug distribution. It emphasizes the crucial role [...] Read more.
Drug-eluting stents (DES) and balloons revolutionize atherosclerosis treatment by targeting hyperplastic tissue responses through effective local drug delivery strategies. This review examines approved and emerging endovascular devices, discussing drug release mechanisms and their impacts on arterial drug distribution. It emphasizes the crucial role of drug delivery in modern cardiovascular care and highlights how device technologies influence vascular behavior based on lesion morphology. The future holds promise for lesion-specific treatments, particularly in the superficial femoral artery, with recent CE-marked devices showing encouraging results. Exciting strategies and new patents focus on local drug delivery to prevent restenosis, shaping the future of interventional outcomes. In summary, as we navigate the ever-evolving landscape of cardiovascular intervention, it becomes increasingly evident that the future lies in tailoring treatments to the specific characteristics of each lesion. By leveraging cutting-edge technologies and harnessing the potential of localized drug delivery, we stand poised to usher in a new era of precision medicine in vascular intervention. Full article
(This article belongs to the Section Pharmaceutical Science)
20 pages, 5960 KiB  
Article
Optimization of the Extraction Conditions of Polyphenols from Red Clover (Trifolium pratense L.) Flowers and Evaluation of the Antiradical Activity of the Resulting Extracts
by Beata Dru?yńska, Jakub ?ukasiewicz, Ewa Majewska and Rafa? Wo?osiak
Antioxidants 2024, 13(4), 414; https://doi.org/10.3390/antiox13040414 (registering DOI) - 28 Mar 2024
Abstract
The purpose of this study was to analyze the effect of the type of extraction solution (water, different concentrations of ethanol), temperature and time on the polyphenol content and antioxidant properties of red clover extracts and the effect of the addition of selected [...] Read more.
The purpose of this study was to analyze the effect of the type of extraction solution (water, different concentrations of ethanol), temperature and time on the polyphenol content and antioxidant properties of red clover extracts and the effect of the addition of selected extracts on the antioxidant properties of enriched blackcurrant beverages. In both the extractions carried out under different conditions and in the enriched beverages, the content of selected polyphenols was determined by HPLC. This study confirmed the significant effect of the alcohol content of the extract, extraction time and temperature on the antioxidant properties of clover extracts. Ethanolic extracts had better antioxidant properties than aqueous extracts. The addition of ethanol extracts had a significant effect on the antioxidant properties of the fortified beverages. Increasing the temperature, time or ethanol content in the extracts mostly resulted in an increase in the total polyphenol content in the obtained extracts. Based on the analysis of the response surface, it was found that for the DPPH radical, the best activity was obtained by extraction for 20 min with a solution of approximately 65% at low temperatures. In the case of the ABTS radical, the best antiradical activity was obtained after extraction for 60 min at 80 °C with a solution of approximately 50% ethanol. It was also found that the use of a solution of approximately 60% ethanol after extraction for 60 min at 80 °C would provide an extract with high antiradical activity against both radicals. Full article
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
20 pages, 3455 KiB  
Article
Calorie Restriction Using High-Fat/Low-Carbohydrate Diet Suppresses Liver Fat Accumulation and Pancreatic Beta-Cell Dedifferentiation in Obese Diabetic Mice
by Xiao Lei, Emi Ishida, Satoshi Yoshino, Shunichi Matsumoto, Kazuhiko Horiguchi and Eijiro Yamada
Nutrients 2024, 16(7), 995; https://doi.org/10.3390/nu16070995 (registering DOI) - 28 Mar 2024
Abstract
In diabetes, pancreatic β-cells gradually lose their ability to secrete insulin with disease progression. β-cell dysfunction is a contributing factor to diabetes severity. Recently, islet cell heterogeneity, exemplified by β-cell dedifferentiation and identified in diabetic animals, has attracted attention as an underlying molecular [...] Read more.
In diabetes, pancreatic β-cells gradually lose their ability to secrete insulin with disease progression. β-cell dysfunction is a contributing factor to diabetes severity. Recently, islet cell heterogeneity, exemplified by β-cell dedifferentiation and identified in diabetic animals, has attracted attention as an underlying molecular mechanism of β-cell dysfunction. Previously, we reported β-cell dedifferentiation suppression by calorie restriction, not by reducing hyperglycemia using hypoglycemic agents (including sodium-glucose cotransporter inhibitors), in an obese diabetic mice model (db/db). Here, to explore further mechanisms of the effects of food intake on β-cell function, db/db mice were fed either a high-carbohydrate/low-fat diet (db-HC) or a low-carbohydrate/high-fat diet (db-HF) using similar calorie restriction regimens. After one month of intervention, body weight reduced, and glucose intolerance improved to a similar extent in the db-HC and db-HF groups. However, β-cell dedifferentiation did not improve in the db-HC group, and β-cell mass compensatory increase occurred in this group. More prominent fat accumulation occurred in the db-HC group livers. The expression levels of genes related to lipid metabolism, mainly regulated by peroxisome proliferator-activated receptor α and γ, differed significantly between groups. In conclusion, the fat/carbohydrate ratio in food during calorie restriction in obese mice affected both liver lipid metabolism and β-cell dedifferentiation. Full article
(This article belongs to the Special Issue Glucose Metabolism in Obese and Diabetic Patients)
24 pages, 1561 KiB  
Article
Spatial Distribution of Soil Macroelements, Their Uptake by Plants, and Green Pea Yield under Strip-till Technology
by Ma?gorzata Szczepanek, Mariusz Piekarczyk and Karolina B?aszczyk
Agronomy 2024, 14(4), 711; https://doi.org/10.3390/agronomy14040711 (registering DOI) - 28 Mar 2024
Abstract
Using conservation tillage to grow crops that enhance soil quality, such as legumes, seems to be one of the best solutions for sustainable agriculture. The field study was conducted to identify the effect of soil cultivation technology and fertilization, via strip-tilling (reduced) vs. [...] Read more.
Using conservation tillage to grow crops that enhance soil quality, such as legumes, seems to be one of the best solutions for sustainable agriculture. The field study was conducted to identify the effect of soil cultivation technology and fertilization, via strip-tilling (reduced) vs. plowing (conventional), on the availability and uptake of NPK and Mg, as well as on the growth of shoots and roots and yield of green peas (Pisum sativum L.). The research was carried out in central Poland (53°05′16.8″ N, 19°06′14.4″ E) over two growing seasons of green peas in 2016 and 2017. Our study has shown that the spatial distribution of macroelements in the soil is influenced by the tillage method. The availability and nutrient uptake by green peas, their growth parameters, and yield were also influenced by the tillage system. However, the effect was observed mainly in the first year of the study, which had less precipitation and higher temperatures. In general, in our study, the strip-till has a positive impact on the nutrient uptake by plants, contributing to longer shoots and roots and higher biomass accumulation, especially in the first part of the growing season. In 2016, with less rainfall, green peas under strip-tilling produced more pods per plant and the yield was higher than under plowing (by 13.8%). In 2017, with higher precipitation, an increase in yield under strip-tilling compared to plowing was also observed (by 9.1%), but this difference was not statistically significant. To sum up, strip-tillage seems to have a positive impact on the spatial distribution of macroelements, growth parameters, and yield of green peas, and can be recommended as a technology for the sustainable production of this crop. Full article
(This article belongs to the Special Issue Applied Research and Extension in Agronomic Soil Fertility Series II)
20 pages, 837 KiB  
Article
Exploring the Untapped Potential of Pine Nut Skin By-Products: A Holistic Characterization and Recycling Approach
by Agata Nolasco, Jonathan Squillante, Salvatore Velotto, Giovanni D’Auria, Pasquale Ferranti, Gianfranco Mamone, Maria Emanuela Errico, Roberto Avolio, Rachele Castaldo, Lucia De Luca, Raffaele Romano, Francesco Esposito and Teresa Cirillo
Foods 2024, 13(7), 1044; https://doi.org/10.3390/foods13071044 (registering DOI) - 28 Mar 2024
Abstract
The increasing population, food demand, waste management concerns, and the search for sustainable alternatives to plastic polymers have led researchers to explore the potential of waste materials. This study focused on a waste of pine nut processing referred to in this paper as [...] Read more.
The increasing population, food demand, waste management concerns, and the search for sustainable alternatives to plastic polymers have led researchers to explore the potential of waste materials. This study focused on a waste of pine nut processing referred to in this paper as pine nut skin. For the first time, its nutritional profile, potential bioactive peptide, contaminants, and morphological structure were assessed. Pine nut skin was composed mainly of carbohydrates (56.2%) and fiber (27.5%). The fat (9.8%) was about 45%, 35%, and 20% saturated, monounsaturated, and polyunsaturated fatty acid, respectively, and Omega-9,-6, and -3 were detected. Notably, oleic acid, known for its health benefits, was found in significant quantities, resembling its presence in pine nut oil. The presence of bioactive compounds such as eicosapentaenoic acid (EPA) and phytosterols further adds to its nutritional value. Some essential elements were reported, whereas most of the contaminants such as heavy metals, polycyclic aromatic hydrocarbons, rare earth elements, and pesticides were below the limit of quantification. Furthermore, the in silico analysis showed the occurrence of potential precursor peptides of bioactive compounds, indicating health-promoting attributes. Lastly, the morphological structural characterization of the pine nut skin was followed by Fourier Transform Infrared and solid-state NMR spectroscopy to identify the major components, such as lignin, cellulose, and hemicellulose. The thermostability of the pine nut skin was monitored via thermogravimetric analysis, and the surface of the integument was analyzed via scanning electron microscopy and volumetric nitrogen adsorption. This information provides a more comprehensive view of the potential uses of pine nut skin as a filler material for biocomposite materials. A full characterization of the by-products of the food chain is essential for their more appropriate reuse. Full article
8 pages, 1402 KiB  
Brief Report
Outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 in a Rural Community Hospital during Omicron Predominance
by Amar Krishna, Julie Tutt, Mehr Grewal, Sheila Bragdon and Suzanne Moreshead
Microorganisms 2024, 12(4), 686; https://doi.org/10.3390/microorganisms12040686 (registering DOI) - 28 Mar 2024
Abstract
Healthcare-associated infections due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has increased since the discovery of the Omicron variant. We describe a SARS-CoV-2 outbreak in the medicine–surgery unit of a rural community hospital at the time of high community transmission of Omicron [...] Read more.
Healthcare-associated infections due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has increased since the discovery of the Omicron variant. We describe a SARS-CoV-2 outbreak in the medicine–surgery unit of a rural community hospital at the time of high community transmission of Omicron variant in our county. The outbreak occurred in the medicine–surgery unit of an 89-bed rural community hospital in northern Maine. The characteristics of the patients and healthcare workers (HCWs) affected by the outbreak are described. Patient and HCW data collected as part of the outbreak investigation were used in this report. The outbreak control measures implemented are also described. A total of 24 people tested positive for SARS-CoV-2 including 11 patients and 13 HCWs. A total of 12 of the 24 (50%) persons were symptomatic, and rhinorrhea was the most common symptom noted (8/12, 67%). None of the symptomatic persons had gastrointestinal symptoms or symptoms of a loss of sense of smell or taste. All HCWs were vaccinated and 8 of the 11 patients were vaccinated. Outbreak control measures in the affected unit included implementation of full PPE (N95 respirators, eye protection, gowns and gloves) during all patient care, serial testing of employees and patients in the affected unit, cohorting positive patients, closing visitation and thorough environmental cleaning including use of ultraviolet (UV) light disinfection. This outbreak exemplifies the high transmissibility of the Omicron variant of SARS-CoV-2. The outbreak occurred despite a well-established infection control program. We noted that serial testing, use of N95 respirators during all patient care and UV disinfection were some of the measures that could be successful in outbreak control. Full article
Show Figures

Figure 1

Full article ">Full article ">Full article ">
14 pages, 484 KiB  
Review
Ablative Treatments for Small Renal Masses and Management of Recurrences: A Comprehensive Review
by Achille Aveta, Vincenzo Iossa, Gianluca Spena, Paolo Conforti, Giovanni Pagano, Fabrizio Dinacci, Paolo Verze, Celeste Manfredi, Matteo Ferro, Francesco Lasorsa, Lorenzo Spirito, Luigi Napolitano, Antonio Tufano, Alessandra Fiorenza, Pierluigi Russo, Fabio Crocerossa, Giuseppe Lucarelli, Sisto Perdonà, Roberto Sanseverino, Salvatore Siracusano, Simone Cilio and Savio Domenico Pandolfoadd Show full author list remove Hide full author list
Life 2024, 14(4), 450; https://doi.org/10.3390/life14040450 (registering DOI) - 28 Mar 2024
Abstract
This review focuses on ablative techniques for small renal masses (SRMs), including radiofrequency ablation (RFA), cryoablation (CA), microwave ablation (MWA), and irreversible electroporation (IRE), and discusses recurrence management. Through an extensive literature review, we outline the procedures, outcomes, and follow-up strategies associated with [...] Read more.
This review focuses on ablative techniques for small renal masses (SRMs), including radiofrequency ablation (RFA), cryoablation (CA), microwave ablation (MWA), and irreversible electroporation (IRE), and discusses recurrence management. Through an extensive literature review, we outline the procedures, outcomes, and follow-up strategies associated with each ablative method. The review provides a detailed examination of these techniques—RFA, CA, MWA, and IRE—elucidating their respective outcomes. Recurrence rates vary among them, with RFA and CA showing comparable rates, MWA demonstrating favorable short-term results, and IRE exhibiting promise in experimental stages. For managing recurrences, various strategies are considered, including active surveillance, re-ablation, or salvage surgery. Surveillance is preferred post-RFA and post-CA, due to slow SRM growth, while re-ablation, particularly with RFA and CA, is deemed feasible without additional complications. Salvage surgery emerges as a viable option for larger or resistant tumors. While ablative techniques offer short-term results comparable to surgery, further research is essential to understand their long-term effects fully. Decisions concerning recurrence management should consider individual and tumor-specific factors. Imaging, notably contrast-enhanced ultrasounds, plays a pivotal role in assessing treatment success, emphasizing the necessity of a multidisciplinary approach for optimal outcomes. The lack of randomized trials highlights the need for further research. Full article
(This article belongs to the Section Medical Research)
13 pages, 642 KiB  
Article
Urea Fertilization Significantly Promotes Nitrous Oxide Emissions from Agricultural Soils and Is Attributed to the Short-Term Suppression of Nitrite-Oxidizing Bacteria during Urea Hydrolysis
by Yiming Jiang, Yueyue Zhu, Weitie Lin and Jianfei Luo
Microorganisms 2024, 12(4), 685; https://doi.org/10.3390/microorganisms12040685 (registering DOI) - 28 Mar 2024
Abstract
The application of urea in agricultural soil significantly boosts nitrous oxide (N2O) emissions. However, the reason for nitrite accumulation, the period of nitrite-oxidizing bacteria (NOB) suppression, and the main NOB species for nitrite removal behind urea fertilization have not been thoroughly [...] Read more.
The application of urea in agricultural soil significantly boosts nitrous oxide (N2O) emissions. However, the reason for nitrite accumulation, the period of nitrite-oxidizing bacteria (NOB) suppression, and the main NOB species for nitrite removal behind urea fertilization have not been thoroughly investigated. In this study, four laboratory microcosm experiments were conducted to simulate urea fertilization in agricultural soils. We found that within 36 h of urea application, nitrite oxidation lagged behind ammonia oxidation, leading to nitrite accumulation and increased N2O emissions. However, after 36 h, NOB activity recovered and then removed nitrite, leading to reduced N2O emissions. Urea use resulted in an N2O emission rate tenfold higher than ammonium. During incubation, Nitrobacter-affiliated NOB growth decreased initially but increased later with urea use, while Nitrospira-affiliated NOB appeared unaffected. Chlorate suppression of NOB lasted longer, increasing N2O emissions. Urease inhibitors effectively reduced N2O emissions by slowing urea hydrolysis and limiting free ammonia production, preventing short-term NOB suppression. In summary, short-term NOB suppression during urea hydrolysis played a crucial role in increasing N2O emissions from agricultural soils. These findings revealed the reasons behind the surge in N2O emissions caused by extensive urea application and provided guidance for reducing N2O emissions in agricultural production processes. Full article
14 pages, 569 KiB  
Article
The Immunometabolic Gene N-Acetylglucosamine Kinase Is Uniquely Involved in the Heritability of Multiple Sclerosis Severity
by Serge Nataf, Marine Guillen and Laurent Pays
Int. J. Mol. Sci. 2024, 25(7), 3803; https://doi.org/10.3390/ijms25073803 (registering DOI) - 28 Mar 2024
Abstract
The clinical severity of multiple sclerosis (MS), an autoimmune disorder of the central nervous system, is thought to be determined by environmental and genetic factors that have not yet been identified. In a recent genome-wide association study (GWAS), a single nucleotide polymorphism (SNP), [...] Read more.
The clinical severity of multiple sclerosis (MS), an autoimmune disorder of the central nervous system, is thought to be determined by environmental and genetic factors that have not yet been identified. In a recent genome-wide association study (GWAS), a single nucleotide polymorphism (SNP), rs10191329, has been associated with MS severity in two large independent cohorts of patients. Different approaches were followed by the authors to prioritize the genes that are transcriptionally regulated by such an SNP. It was concluded that the identified SNP regulates a group of proximal genes involved in brain resilience and cognitive abilities rather than immunity. Here, by conducting an alternative strategy for gene prioritization, we reached the opposite conclusion. According to our re-analysis, the main target of rs10191329 is N-Acetylglucosamine Kinase (NAGK), a metabolic gene recently shown to exert major immune functions via the regulation of the nucleotide-binding oligomerization domain-containing protein 2 (NOD2) pathway. To gain more insights into the immunometabolic functions of NAGK, we analyzed the currently known list of NAGK protein partners. We observed that NAGK integrates a dense network of human proteins that are involved in glucose metabolism and are highly expressed by classical monocytes. Our findings hold potentially major implications for the understanding of MS pathophysiology. Full article
11 pages, 405 KiB  
Article
Combined Permutation Tests for Pairwise Comparison of Scale Parameters Using Deviances
by Scott J. Richter and Melinda H. McCann
Stats 2024, 7(2), 350-360; https://doi.org/10.3390/stats7020021 (registering DOI) - 28 Mar 2024
Abstract
Nonparametric combinations of permutation tests for pairwise comparison of scale parameters, based on deviances, are examined. Permutation tests for comparing two or more groups based on the ratio of deviances have been investigated, and a procedure based on Higgins’ RMD statistic was found [...] Read more.
Nonparametric combinations of permutation tests for pairwise comparison of scale parameters, based on deviances, are examined. Permutation tests for comparing two or more groups based on the ratio of deviances have been investigated, and a procedure based on Higgins’ RMD statistic was found to perform well, but two other tests were sometimes more powerful. Thus, combinations of these tests are investigated. A simulation study shows a combined test can be more powerful than any single test. Full article
(This article belongs to the Section Statistical Methods)
25 pages, 2899 KiB  
Article
Effect of Turbulent Wind Conditions on the Dynamic Characteristics of a Herringbone Planetary Gear System of a Wind Turbine
by Wei-qiang Zhao, Wenhui Zhao, Jie Liu and Na Yang
Machines 2024, 12(4), 227; https://doi.org/10.3390/machines12040227 (registering DOI) - 28 Mar 2024
Abstract
Due to complex environmental factors, the gear transmission systems of wind turbines are continuously affected by large torque load excitation with periodic and random properties. This paper shares the load-sharing and dynamic characteristics of a herringbone planetary gear system applied in a wind [...] Read more.
Due to complex environmental factors, the gear transmission systems of wind turbines are continuously affected by large torque load excitation with periodic and random properties. This paper shares the load-sharing and dynamic characteristics of a herringbone planetary gear system applied in a wind turbine. The gear dynamic model is established using a typical lumped parameter method, in which the nonlinear transmission errors of the gear pairs and left and right-side coupling stiffness of the herringbone gears are included. With the help of the blade element momentum theory, the precise calculation of the hub load of the wind turbine, which is the external excitation of the gear system, is implemented, in which the wind shear, tower shadow, turbulent effect, and tip loss correction are taken into consideration. The nonlinear dynamic characteristics of the system are obtained using the Runge-Kutta method and then discussed. The results show that the turbulent effect plays a major role in the impact on the load-sharing characteristics, and a reasonable set of the support stiffness of rotational components can improve the load-sharing characteristics of the system. The purpose of this research is to provide some useful references in numerical modelling and methods for designers and researchers of wind turbine transmission systems. Full article
(This article belongs to the Section Turbomachinery)
12 pages, 568 KiB  
Article
Paraneoplastic Syndromes in Hepatocellular Carcinoma, Epidemiology, and Survival: A Retrospective Seven Years Study
by Calin Burciu, Roxana Sirli, Renata Bende, Deiana Vuletici, Bogdan Miutescu, Tudor Moga, Felix Bende, Alina Popescu, Ioan Sporea, Oana Koppandi, Eftimie Miutescu, Dana Iovanescu and Mirela Danila
Medicina 2024, 60(4), 552; https://doi.org/10.3390/medicina60040552 (registering DOI) - 28 Mar 2024
Abstract
Background and Objectives: Liver cancer poses a significant global health threat, ranking among the top three causes of cancer-related deaths. Patients with hepatocellular carcinoma (HCC) often present with symptoms associated with neoplasms or unusual clinical features such as paraneoplastic syndromes (PNS), including hypoglycemia, [...] Read more.
Background and Objectives: Liver cancer poses a significant global health threat, ranking among the top three causes of cancer-related deaths. Patients with hepatocellular carcinoma (HCC) often present with symptoms associated with neoplasms or unusual clinical features such as paraneoplastic syndromes (PNS), including hypoglycemia, hypercholesterolemia, thrombocytosis, and erythrocytosis. Our study aimed to investigate the prevalence, clinical characteristics, and survival outcomes associated with PNS in HCC patients and assess each PNS’s impact on patient survival. Materials and Methods: We conducted a retrospective analysis of PNS clinical features and survival among consecutive HCC patients diagnosed at our department over seven years, comparing them with HCC patients without PNS. The study involved a retrospective data evaluation from 378 patients diagnosed with HCC between January 2016 and October 2023. Results: We obtained a PNS prevalence of 25.7%, with paraneoplastic hypercholesterolemia at 10.9%, hypoglycemia at 6.9%, erythrocytosis at 4.5%, and thrombocytosis at 3.4%. Patients with PNS tended to be younger and predominantly male. Multivariate analysis revealed a strong correlation between PNS and levels of alpha-fetoprotein and tumor size, with diabetes also showing a significant statistical association (p < 0.05). Subgroup analysis based on specific paraneoplastic syndromes demonstrated shorter survival in patients with PNS, albeit without significant statistical differences, except for hypoglycemia (p < 0.0001). Matched analysis indicated a shorter survival rate for patients with PNS, although no significant statistical differences were observed. Conclusions: PNS are frequently observed in HCC cases and are associated with unfavorable prognoses and decreased survival rates due to their correlation with increased tumor burdens. However, they do not independently predict poor survival. The impact of individual PNS on HCC prognosis varies. Full article
Show Figures

Figure 1

Full article ">
11 pages, 532 KiB  
Article
Quality of Life after Treatment for Pelvic Organ Prolapse in Real-World Study: Recommendations, Vaginal Pessary, and Surgery
by Dominyka Man?inskien?, Migl? Mik?nait?, Mark Barakat, Justina Ka?erauskien?, Dalia Regina Railait?, Laima Maleckien?, Arnoldas Bartusevi?ius and Egl? Bartusevi?ien?
Medicina 2024, 60(4), 547; https://doi.org/10.3390/medicina60040547 (registering DOI) - 28 Mar 2024
Abstract
Background and Objectives: Pelvic organ prolapse (POP) is a common condition in women, with its prevalence increasing with age, and can significantly impact the quality of life (QOL) of many individuals. The objective of this study was to assess the overall improvement, quality [...] Read more.
Background and Objectives: Pelvic organ prolapse (POP) is a common condition in women, with its prevalence increasing with age, and can significantly impact the quality of life (QOL) of many individuals. The objective of this study was to assess the overall improvement, quality of life, and continuation of primary treatment for POP over a 24-month period in a real-world setting. Materials and Methods: This is a prospective, observational, follow-up study of women with symptomatic POP who, as a primary treatment, opted for recommendations (lifestyle changes and pelvic floor muscle training), pessary therapy, or surgery. The primary outcome measure was a subjective improvement at the 24-month follow-up, measured with the Patient Global Impression of Improvement (PGI-I) scale. Secondary outcome measures were the continuation rate of the primary treatment method, reason for discontinuation, and the quality of life evaluated with the P-QoL questionnaire. Results: We included 137 women, with 45 women (32.8%) in the recommendations group, 39 (28.5%) in the pessary group, and 53 women (38.7%) in the surgery group. After 24 months, surgery, in comparison with pessary treatment and recommendations, resulted in significantly more women reporting a subjective improvement: 89.6%, 66.7%, and 22.9% (p < 0.001), respectively. Overall, 52% of women from the recommendations group and 36.4% from the pessary group switched to another treatment or discontinued the primary treatment within 24 months. However, women who continued the primary treatment, pessary use, and surgery showed similar subjective improvements (90.5% and 89.6%, respectively) and quality-of-life improvement. Conclusions: The chance of significant improvement was higher following surgery. However, after 24 months, both vaginal pessaries and surgery showed an important quality-of life improvement and can be proposed as primary treatment methods for pelvic organ prolapse. Full article
Show Figures

Figure 1

Full article ">
17 pages, 567 KiB  
Article
GSTM1 and GSTP1 Polymorphisms Affect Outcome in Colorectal Adenocarcinoma
by Milica Stojkovic Lalosevic, Vesna Coric, Tatjana Pekmezovic, Tatjana Simic, Aleksandra Pavlovic Markovic and Marija Pljesa Ercegovac
Medicina 2024, 60(4), 553; https://doi.org/10.3390/medicina60040553 (registering DOI) - 28 Mar 2024
Abstract
Background and Objectives: Despite improvements in screening programs, a large number of patients with colorectal cancer (CRC) are diagnosed in an advanced disease stage. Previous investigations imply that glutathione transferases (GSTs) might be associated with the development and progression of CRC. Moreover, [...] Read more.
Background and Objectives: Despite improvements in screening programs, a large number of patients with colorectal cancer (CRC) are diagnosed in an advanced disease stage. Previous investigations imply that glutathione transferases (GSTs) might be associated with the development and progression of CRC. Moreover, the detoxification mechanism of oxaliplatin, which represents the first line of treatment for advanced CRC, is mediated via certain GSTs. The aim of this study was to evaluate the significance of certain GST genetic variants on CRC prognosis and the efficacy of oxaliplatin-based treatment. Materials and Methods: This prospective study included 523 patients diagnosed with CRC in the period between 2014 and 2016, at the Digestive Surgery Clinic, University Clinical Center of Serbia, Belgrade. Patients were followed for a median of 43.47 ± 17.01 months (minimum 1–63 months). Additionally, 109 patients with advanced disease, after surgical treatment, received FOLFOX6 treatment as a first-line therapy between 2014 and 2020. The Kaplan–Meier method was used to analyze cumulative survival, and the Cox proportional hazard regression model was used to study the effects of different GST genotypes on overall survival. Results: Individuals with the GSTM1-null genotype and the GSTP1 IleVal+ValVal (variant) genotype had significantly shorter survival when compared to referent genotypes (GSTM1-active and GSTP1 IleIle) (log-rank: p = 0.001). Moreover, individuals with the GSTM1-null genotype who received 5-FU-based treatment had statistically significantly shorter survival when compared to individuals with the GSTM1-active genotype (log-rank: p = 0.05). Conclusions: Both GSTM1-null and GSTP1 IleVal+ValVal (variant) genotypes are associated with significantly shorter survival in CRC patients. What is more, the GSTM1-null genotype is associated with shorter survival in patients receiving FOLOFOX6 treatment. Full article
11 pages, 613 KiB  
Article
A Comparison of Different Strength Measurement in Taekwondo: Herman Trainer, Manual Tester, and Standing Long Jump
by Ay?e Hazal Boyanm??, ?nci Kesilmi?, Manolya Ak?n, Buse Yilmaz, A?ina Uslular, Yesim Karac Ocal and Hulya Andre
Medicina 2024, 60(4), 550; https://doi.org/10.3390/medicina60040550 (registering DOI) - 28 Mar 2024
Abstract
Background and Objective: The accurate prediction of strength development relies on identifying the most appropriate measurement methods. This study compared diverse strength measurement techniques to assess their effectiveness in predicting strength development. Participants were taekwondo athletes competing at the red–black belt level [...] Read more.
Background and Objective: The accurate prediction of strength development relies on identifying the most appropriate measurement methods. This study compared diverse strength measurement techniques to assess their effectiveness in predicting strength development. Participants were taekwondo athletes competing at the red–black belt level or above. Methods: Technical striking forces (palding, dollyeo chagi, dwit chagi, and yeop chagi) were measured using a Herman Digital Trainer fixed to a striking stand. Quadriceps and hamstring strength were assessed with a Lafayette force measuring device. Explosive leg strength was evaluated through a standing long jump test, normalized for leg length. The Pearson correlation coefficient was used to examine relationships between measurement methods. Results: The standing long jump test showed no significant correlation with other strength assessments. A moderate positive correlation was found between Herman digital trainer measurements and Lafayette digital hand-held dynamometer results. A high positive correlation (r = 0.736, p < 0.001) emerged between hamstring strength and palding chagi technical strike force results. Technical strike kicks showed a significant positive correlation with each other and, also, a right foot–left foot correlation was observed. Conclusions: It was concluded that the standing long jump test, which was shown as one of the explosive leg strength measurement methods in field studies as an alternative to laboratory tests, did not correlate with other strength tests; therefore, this test is weak and insufficient to predict strength skills in taekwondo. In addition, this study showed that the hamstring muscle was more predictive in the measurement of technical strength. In future studies, it might be more useful to measure hamstring muscle strength or technical kick strength instead of a standing long jump field test. Full article
11 pages, 1385 KiB  
Review
Successful Needle Aspiration of a Traumatic Pneumothorax: A Case Report and Literature Review
by Giuseppe Bettoni, Silvia Gheda, Michele Altomare, Stefano Piero Bernardo Cioffi, Davide Ferrazzi, Michela Cazzaniga, Luca Bonacchini, Stefania Cimbanassi and Paolo Aseni
Medicina 2024, 60(4), 548; https://doi.org/10.3390/medicina60040548 (registering DOI) - 28 Mar 2024
Abstract
Traumatic pneumothorax (PTX) occurs in up to 50% of patients with severe polytrauma and chest injuries. Patients with a traumatic PTX with clinical signs of tension physiology and hemodynamic instability are typically treated with an urgent decompressive thoracostomy, tube thoracostomy, or needle decompression. [...] Read more.
Traumatic pneumothorax (PTX) occurs in up to 50% of patients with severe polytrauma and chest injuries. Patients with a traumatic PTX with clinical signs of tension physiology and hemodynamic instability are typically treated with an urgent decompressive thoracostomy, tube thoracostomy, or needle decompression. There is recent evidence that non-breathless patients with a hemodynamically stable traumatic PTX can be managed conservatively through observation or a percutaneous pigtail catheter. We present here a 52-year-old woman who presented to the emergency department with a 55 mm traumatic PTX. Following aspiration of 1500 mL of air, a clinical improvement was immediately observed, allowing the patient to be discharged shortly thereafter. In hemodynamically stable patients with a post-traumatic PTX, without specific risk factors or oxygen desaturation, observation or simple needle aspiration can be a reasonable approach. Although the recent medical literature supports conservative management of small traumatic PTXs, guidelines are lacking for hemodynamically stable patients with a significantly large PTX. This case report documents our successful experience with needle aspiration in such a setting of large traumatic PTX. We aimed in this article to review the available literature on needle aspiration and conservative treatment of traumatic pneumothorax. A total of 12 studies were selected out of 190 articles on traumatic PTX where conservative treatment and chest tube decompression were compared. Our case report offers a novel contribution by illustrating the successful resolution of a sizable pneumothorax through needle aspiration, suggesting that even a large PTX in a hemodynamically stable patient, without other risk conditions, can be successfully treated conservatively with simple needle aspiration in order to avoid tube thoracostomy complications. Full article
(This article belongs to the Section Emergency Medicine)
Show Figures

Figure 1

Full article ">Full article ">
14 pages, 7898 KiB  
Review
In-Stent Restenosis Overview: From Intravascular Imaging to Optimal Percutaneous Coronary Intervention Management
by Neda Shafiabadi Hassani, Lucas Carlini Ogliari, Pedro Rafael Vieira de Oliveira Salerno, Gabriel Tensol Rodrigues Pereira, Marcelo Harada Ribeiro and Luis Augusto Palma Dallan
Medicina 2024, 60(4), 549; https://doi.org/10.3390/medicina60040549 (registering DOI) - 28 Mar 2024
Abstract
Despite ongoing progress in stent technology and deployment techniques, in-stent restenosis (ISR) still remains a major issue following percutaneous coronary intervention (PCI) and accounts for 10.6% of all interventions in the United States. With the continuous rise in ISR risk factors such as [...] Read more.
Despite ongoing progress in stent technology and deployment techniques, in-stent restenosis (ISR) still remains a major issue following percutaneous coronary intervention (PCI) and accounts for 10.6% of all interventions in the United States. With the continuous rise in ISR risk factors such as obesity and diabetes, along with an increase in the treatment of complex lesions with high-risk percutaneous coronary intervention (CHIP), a substantial growth in ISR burden is expected. This review aims to provide insight into the mechanisms, classification, and management of ISR, with a focus on exploring innovative approaches to tackle this complication comprehensively, along with a special section addressing the approach to complex calcified lesions. Full article
(This article belongs to the Special Issue Cutting-Edge Research in Percutaneous Coronary Intervention)
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
11 pages, 421 KiB  
Article
Treatment of Osteoporosis in Men on Androgen Deprivation Therapy in Japan
by Hanako Nishimoto, Atsuyuki Inui, Yutaka Mifune, Kohei Yamaura, Yukari Bando, Yasuyoshi Okamura, Takuto Hara, Tomoaki Terakawa and Ryosuke Kuroda
Medicina 2024, 60(4), 551; https://doi.org/10.3390/medicina60040551 (registering DOI) - 28 Mar 2024
Abstract
Background and Objectives: Androgen deprivation therapy (ADT) for prostate cancer has greatly improved treatment outcomes. As patient survival rates have increased, reports of decreased bone density and increased bone fractures as side effects of ADT have emerged. The prevalence of osteoporosis in Japanese [...] Read more.
Background and Objectives: Androgen deprivation therapy (ADT) for prostate cancer has greatly improved treatment outcomes. As patient survival rates have increased, reports of decreased bone density and increased bone fractures as side effects of ADT have emerged. The prevalence of osteoporosis in Japanese men was 4.6%. The purpose of this study was to evaluate the effect of osteoporosis treatment in prostate cancer patients who underwent ADT in Japan. Materials and Methods: The subjects were 33 male patients who had undergone ADT for prostate cancer, who were noted to have decreased bone density. Mean age was 76.2 ± 7.7 years (64–87). Medications included vitamin D in one case, bisphosphonates (BP) in 27 cases, and denosumab in five cases. The evaluation method examined the rate of change in bone mineral density (BMD) before osteoporosis treatment and 1 year after. For comparison, a group without osteoporosis treatment intervention (n = 33) was selected, and matched for prostate cancer treatment and age. The rate of change in trabecular bone score (TBS) was also calculated. Results: The percentage changes in BMD before and 1 year after treatment were as follows: lumbar spine, 7.1 ± 5.8% in the treatment group versus −3.9 ± 4.1% in the no treatment group; femoral neck, 5.5 ± 6.2% in the treatment group versus −0.9 ± 3.9% in the no treatment group; total femur, 6.6 ± 6.4% in the treatment group versus the no treatment group which was −1.7 ± 3.2%. In all cases, there was a clear significant difference (p < 0.01). The percent change in TBS was further calculated in the same manner. There was no significant difference between the two groups: +1.7 ± 3.8% in the treated group versus +0.3 ± 4.1% in the untreated group. Conclusions: Osteoporosis treatment in Japanese patients with prostate cancer on ADT therapy was found to significantly increase BMD compared to the untreated group. BP and denosumab were found to be very effective in increasing BMD. Full article
(This article belongs to the Special Issue Update on Osteoporosis)
19 pages, 13616 KiB  
Article
Synthetic-to-Real Composite Semantic Segmentation in Additive Manufacturing
by Aliaksei Petsiuk, Harnoor Singh, Himanshu Dadhwal and Joshua M. Pearce
J. Manuf. Mater. Process. 2024, 8(2), 66; https://doi.org/10.3390/jmmp8020066 (registering DOI) - 28 Mar 2024
Abstract
The application of computer vision and machine learning methods for semantic segmentation of the structural elements of 3D-printed products in the field of additive manufacturing (AM) can improve real-time failure analysis systems and potentially reduce the number of defects by providing additional tools [...] Read more.
The application of computer vision and machine learning methods for semantic segmentation of the structural elements of 3D-printed products in the field of additive manufacturing (AM) can improve real-time failure analysis systems and potentially reduce the number of defects by providing additional tools for in situ corrections. This work demonstrates the possibilities of using physics-based rendering for labeled image dataset generation, as well as image-to-image style transfer capabilities to improve the accuracy of real image segmentation for AM systems. Multi-class semantic segmentation experiments were carried out based on the U-Net model and the cycle generative adversarial network. The test results demonstrated the capacity of this method to detect such structural elements of 3D-printed parts as a top (last printed) layer, infill, shell, and support. A basis for further segmentation system enhancement by utilizing image-to-image style transfer and domain adaptation technologies was also considered. The results indicate that using style transfer as a precursor to domain adaptation can improve real 3D printing image segmentation in situations where a model trained on synthetic data is the only tool available. The mean intersection over union (mIoU) scores for synthetic test datasets included 94.90% for the entire 3D-printed part, 73.33% for the top layer, 78.93% for the infill, 55.31% for the shell, and 69.45% for supports. Full article
(This article belongs to the Special Issue Smart and Advanced Manufacturing)
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
20 pages, 2374 KiB  
Article
Comprehensive Empirical Modeling of Shear Strength Prediction in Reinforced Concrete Deep Beams
by Eyad K. Sayhood, Nisreen S. Mohammed, Salam J. Hilo and Salih S. Salih
Infrastructures 2024, 9(4), 67; https://doi.org/10.3390/infrastructures9040067 (registering DOI) - 28 Mar 2024
Abstract
This paper presents a thorough investigation into the shear strength capacity of reinforced concrete deep beams, with a focus on improving predictive accuracy beyond existing code provisions. Analyzing 198 deep beams from 15 investigations, this study considers parameters such as the concrete compressive [...] Read more.
This paper presents a thorough investigation into the shear strength capacity of reinforced concrete deep beams, with a focus on improving predictive accuracy beyond existing code provisions. Analyzing 198 deep beams from 15 investigations, this study considers parameters such as the concrete compressive strength (f′c), the shear span-to-effective depth ratio (av/d), and reinforcement ratios (ps, pv, and ph). Introducing a novel predictive model, this study conducts a rigorous evaluation using a nonlinear regression analysis and statistical metrics (MAE, RMSE, and R2). The proposed model demonstrates a significant reduction in the coefficient of variation (CV) to 27.08%, surpassing existing codes’ limitations. Comparative analyses highlight the model’s robustness, revealing an improved convergence of data points and minimal sensitivity to variations in key parameters. The findings suggest that the proposed model offers enhanced predictive accuracy across diverse scenarios, making it a valuable tool for structural engineers. This research contributes to advancing the understanding of shear strength in reinforced concrete deep beams, offering a reliable and versatile predictive model with implications for refining design methodologies and enhancing safety with the efficiency of structural systems. Full article
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
14 pages, 377 KiB  
Review
The Relationship between Posture and Muscle Tensive Dysphonia in Teachers: A Systematic Scoping Review
by Roberta Franzone, Luca Petrigna, Daniele Signorelli and Giuseppe Musumeci
J. Funct. Morphol. Kinesiol. 2024, 9(2), 60; https://doi.org/10.3390/jfmk9020060 (registering DOI) - 28 Mar 2024
Abstract
Teachers usually present work-related pain such as neck pain. Their posture could be the cause of these problems; indeed, it is often a sway-back posture. Furthermore, teachers can also experience problems with their voice such as dysphonia, specifically muscle tension dysphonia (MTD). This [...] Read more.
Teachers usually present work-related pain such as neck pain. Their posture could be the cause of these problems; indeed, it is often a sway-back posture. Furthermore, teachers can also experience problems with their voice such as dysphonia, specifically muscle tension dysphonia (MTD). This scoping review aims to find the correlation between teachers’ posture and MTD. It also studies how a posture-based treatment can influence this disorder. Randomized controlled trials, controlled clinical trials, prospective cohort studies, and cross-sectional studies that considered the relationship between posture and MTD and that included teachers in their sample. The search led to an initial number of 396 articles; after the screening process, a final number of eight articles were included. A total of 303 patients were analyzed and all showed altered alignment of the head around the cervical spine with hypertonus of the cricothyroid, suprahyoid, and sternocleidomastoid muscles. Although MTD is a disorder with a multifactorial etiology, the articles revealed a correlation between posture and MTD related to a forward protraction of the cervical spine with a hypertonus of the laryngeal and hyoid musculature. This study also detected that an intervention in posture could reduce vocal disorders. Full article
(This article belongs to the Section Functional Anatomy and Musculoskeletal System)
22 pages, 1870 KiB  
Article
Numerical Study of Cavitation Characteristics through Butterfly Valve under Different Regulation Conditions
by Guang Zhang, Runhua Hu, Dapeng Yin, Desheng Chen, Haolin Zhou and Zhe Lin
Processes 2024, 12(4), 686; https://doi.org/10.3390/pr12040686 (registering DOI) - 28 Mar 2024
Abstract
Butterfly valves are widely used in the pipeline transportation industry due to their safety and reliability, as well as their low manufacturing and operation costs. Cavitation is a common phenomenon in the butterfly valve that can lead to serious damage to a valve’s [...] Read more.
Butterfly valves are widely used in the pipeline transportation industry due to their safety and reliability, as well as their low manufacturing and operation costs. Cavitation is a common phenomenon in the butterfly valve that can lead to serious damage to a valve’s components. Therefore, it is important to investigate the generation and evolution of cavitation in butterfly valves. In this study, LES and the Zwart model were used as the turbulence and cavitation models, respectively, to simulate cavitation through a butterfly valve. The influence of the valve opening degree and inlet flow velocity on dynamic cavitation through the butterfly valve were studied. Furthermore, the cavitated flow field was examined, along with the performance coefficients of the butterfly valve. With the increase in the incoming flow velocity, the high-speed jet zone over a large-range and low-pressure zone appeared inside the downstream of butterfly valve, which affected its stability and the cavitation generation through the valve. Furthermore, the flow coefficient decreased with the increase in vapor volume. In addition, the results indicated that cavitation was more easily induced for smaller valve opening degrees, and the interaction between cavitation and solid walls was stronger. Due to the existence of cavitation, the flow characteristics of butterfly valves are seriously affected. Full article
(This article belongs to the Section Process Control and Monitoring)
15 pages, 2187 KiB  
Article
Effect of Photodynamic Therapy with the Photosensitizer Methylene Blue on Cerebral Endotheliocytes In Vitro
by Vladimir I. Makarov, Alexey S. Skobeltsin, Anton S. Averchuk, Arseniy K. Berdnikov, Milana V. Chinenkova, Alla B. Salmina and Victor B. Loschenov
Photonics 2024, 11(4), 316; https://doi.org/10.3390/photonics11040316 (registering DOI) - 28 Mar 2024
Abstract
Background: Microvessels in tumor tissue play a crucial role in meeting the metabolic needs of transformed cells, controlling the entry of xenobiotics into tumor tissue, and regulating local inflammation that promotes metastasis. Methylene blue has photosensitizing properties and can also affect dysfunctional mitochondria. [...] Read more.
Background: Microvessels in tumor tissue play a crucial role in meeting the metabolic needs of transformed cells, controlling the entry of xenobiotics into tumor tissue, and regulating local inflammation that promotes metastasis. Methylene blue has photosensitizing properties and can also affect dysfunctional mitochondria. Methods: The study was performed on the primary culture of CECs. The cells underwent photodynamic treatment through 660 nm laser irradiation at a power density of 300 mW/cm2. The MTT, TMRE, and TUNEL assays were used to assess the survival, redox metabolism, mitochondrial activity, and apoptosis of CECs. Additionally, the metabolic activity of cells was evaluated using FLIM by measuring the fluorescence lifetime of NADH and FAD. Results: When CECs were incubated with MB, there was an increase in mitochondrial activity that was dependent on the concentration of MB. Additionally, mitochondrial activity increased when the CECs were exposed to 660 nm laser irradiation at an energy dose of up to 5 J/cm2. Following PDT, a slight shift towards oxidative phosphorylation was observed. Conclusions: In vitro application of MB accumulation or laser irradiation causes a shift in the redox status of CECs towards increased reducing activity, without causing any cell damage. However, the combined action of PS and laser radiation has the opposite effect on the redox status of cells, resulting in an increase in the oxidized form of FAD. Full article
(This article belongs to the Special Issue Phototheranostics: Science and Applications)

Open Access Journals

Browse by Indexing Browse by Subject Selected Journals
Back to TopTop
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news