Advancing Open Science
for more than 25 years
Supporting academic communities
since 1996
 
20 pages, 1169 KiB  
Article
The Association between Glymphatic System and Perivascular Macrophages in Brain Waste Clearance
by Jasleen Kaur, Edward D. Boyd, Guangliang Ding, Li Zhang, Hao Luo, Qingjiang Li, Lian Li, Min Wei, Julie Landschoot-Ward, Michael Chopp, Zhenggang Zhang and Quan Jiang
Diagnostics 2024, 14(7), 731; https://doi.org/10.3390/diagnostics14070731 (registering DOI) - 29 Mar 2024
Abstract
The glymphatic system suggests the convective bulk flow of cerebrospinal fluid (CSF) through perivascular spaces and the interstitial spaces of the brain parenchyma for the rapid removal of toxic waste solutes from the brain. However, the presence of convective bulk flow within the [...] Read more.
The glymphatic system suggests the convective bulk flow of cerebrospinal fluid (CSF) through perivascular spaces and the interstitial spaces of the brain parenchyma for the rapid removal of toxic waste solutes from the brain. However, the presence of convective bulk flow within the brain interstitial spaces is still under debate. We first addressed this argument to determine the involvement of the glymphatic system in brain waste clearance utilizing contrast-enhanced 3D T1-weighted imaging (T1WI), diffusion tensor imaging (DTI), and confocal microscopy imaging. Furthermore, perivascular macrophages (PVMs), which are immune cells located within perivascular spaces, have not been thoroughly explored for their association with the glymphatic system. Therefore, we investigated tracer uptake by PVMs in the perivascular spaces of both the arteries/arterioles and veins/venules and the potential association of PVMs in assisting the glymphatic system for interstitial waste clearance. Our findings demonstrated that both convective bulk flow and diffusion are responsible for the clearance of interstitial waste solutes from the brain parenchyma. Furthermore, our results suggested that PVMs may play an important function in glymphatic system-mediated interstitial waste clearance. The glymphatic system and PVMs could be targeted to enhance interstitial waste clearance in patients with waste-associated neurological conditions and aging. Full article
(This article belongs to the Section Medical Imaging and Theranostics)
11 pages, 3009 KiB  
Article
Procalcitonin Detection Using Immunomagnetic Beads-Mediated Surface-Enhanced Raman Spectroscopy
by Jiayue Huang, Dagan Zhang, Yan Zu and Lexiang Zhang
Biosensors 2024, 14(4), 164; https://doi.org/10.3390/bios14040164 (registering DOI) - 29 Mar 2024
Abstract
The early detection of procalcitonin (PCT) is crucial for diagnosing bacterial infections due to its high sensitivity and specificity. While colloidal gold colorimetric and immune-chemiluminescence methods are commonly employed in clinical detection, the former lacks sensitivity, and the latter faces challenges with a [...] Read more.
The early detection of procalcitonin (PCT) is crucial for diagnosing bacterial infections due to its high sensitivity and specificity. While colloidal gold colorimetric and immune-chemiluminescence methods are commonly employed in clinical detection, the former lacks sensitivity, and the latter faces challenges with a brief luminescence process and an elevated background. Here, we introduce a novel approach for the quantitative analysis of PCT using surface-enhanced Raman spectroscopy (SERS), leveraging the enhanced properties of metal nanoparticles. Simultaneously, we employed a magnetic nanoparticle coating and surface biofunctionalization modification to immobilize PCT-trapping antibodies, creating the required immune substrates. The resulting magnetic nanoparticles and antibody complexes, acting as carriers and recognition units, exhibited superparamagnetism and the specific recognition of biomarkers. Then, this complex efficiently underwent magnetic separation with an applied magnetic field, streamlining the cumbersome steps of traditional ELISA and significantly reducing the detection time. In conclusion, the exploration of immunomagnetic bead detection technology based on surface-enhanced Raman spectroscopy holds crucial practical significance for the sensitive detection of PCT. Full article
(This article belongs to the Section Biosensors and Healthcare)
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
17 pages, 782 KiB  
Article
Synergetic Effects of Aloe Vera Extract with Trimethylglycine for Targeted Aquaporin 3 Regulation and Long-Term Skin Hydration
by Viktor Filatov, Anna Sokolova, Natalya Savitskaya, Mariya Olkhovskaya, Andrey Varava, Egor Ilin and Elizaveta Patronova
Molecules 2024, 29(7), 1540; https://doi.org/10.3390/molecules29071540 (registering DOI) - 29 Mar 2024
Abstract
Aquaporin 3 (AQP3) channels are tetrameric membrane-bound channels that facilitate the transport of water and other small solutes across cell membranes in the skin. Decreased AQP3 expression is associated with skin dryness, skin aging, psoriasis, and delayed wound healing. Thus, our study focused [...] Read more.
Aquaporin 3 (AQP3) channels are tetrameric membrane-bound channels that facilitate the transport of water and other small solutes across cell membranes in the skin. Decreased AQP3 expression is associated with skin dryness, skin aging, psoriasis, and delayed wound healing. Thus, our study focused on a novel combination based on Aloe barbadensis leaf extract and trimethylglycine for targeted AQP3 regulation in skin keratinocytes and deep skin moisturization. Firstly, a dose-finding cytotoxicity assay of the selected substances was performed with a 2,5-diphenyl-2H-tetrazolium bromide (MTT) indicator on HaCaT cells. The substances’ ability to increase the amount of AQP3 in keratinocytes was evaluated in a keratinocyte cell culture by means of ELISA. Additionally, the deep skin hydration effect was confirmed in clinical research with healthy volunteers. According to the results, the maximum tolerated doses providing viability at 70% (MTDs) values for Aloe barbadensis leaf extract and trimethylglycine were 24.50% and 39.00%, respectively. Following the research and development, a complex based on Aloe barbadensis leaf extract and trimethylglycine in a 1:1 mass ratio exhibited a good cytotoxicity profile, with an MTDs value of 37.90%. Furthermore, it was shown that the combination had a clear synergetic effect and significantly increased AQP3 by up to 380% compared to the negative control and glyceryl glucoside (p < 0.001). It was clinically confirmed that the developed shower gel containing Aloe barbadensis leaf extract and trimethylglycine safely improved skin hydration after one use and over 28 days. Thus, this novel plant-based combination has promising potential for AQP3 regulation in the skin epidermis and a role in the development of dermatological drugs for the treatment of skin xerosis and atopic-related conditions. Full article
(This article belongs to the Special Issue Natural Products and Analogues with Promising Biological Profiles)
Show Figures

Graphical abstract

Full article ">
13 pages, 772 KiB  
Review
Assessing Animal Models to Study Impaired and Chronic Wounds
by Shayan Saeed and Manuela Martins-Green
Int. J. Mol. Sci. 2024, 25(7), 3837; https://doi.org/10.3390/ijms25073837 (registering DOI) - 29 Mar 2024
Abstract
Impaired healing wounds do not proceed through the normal healing processes in a timely and orderly manner, and while they do eventually heal, their healing is not optimal. Chronic wounds, on the other hand, remain unhealed for weeks or months. In the US [...] Read more.
Impaired healing wounds do not proceed through the normal healing processes in a timely and orderly manner, and while they do eventually heal, their healing is not optimal. Chronic wounds, on the other hand, remain unhealed for weeks or months. In the US alone, chronic wounds impact ~8.5 million people and cost ~USD 28–90 billion per year, not accounting for the psychological and physical pain and emotional suffering that patients endure. These numbers are only expected to rise in the future as the elderly populations and the incidence of comorbidities such as diabetes, hypertension, and obesity increase. Over the last few decades, scientists have used a variety of approaches to treat chronic wounds, but unfortunately, to date, there is no effective treatment. Indeed, while there are thousands of drugs to combat cancer, there is only one single drug approved for the treatment of chronic wounds. This is in part because wound healing is a very complex process involving many phases that must occur sequentially and in a timely manner. Furthermore, models that fully mimic human chronic wounds have not been developed. In this review, we assess various models currently being used to study the biology of impaired healing and chronic non-healing wounds. Among them, this paper also highlights one model which shows significant promise; this model uses aged and obese db/db−/− mice and the chronic wounds that develop show characteristics of human chronic wounds that include increased oxidative stress, chronic inflammation, damaged microvasculature, abnormal collagen matrix deposition, a lack of re-epithelialization, and the spontaneous development of multi-bacterial biofilm. We also discuss how important it is that we continue to develop chronic wound models that more closely mimic those of humans and that can be used to test potential treatments to heal chronic wounds. Full article
(This article belongs to the Special Issue Cellular and Molecular Mechanisms on Wound Healing)
18 pages, 4339 KiB  
Article
Microstructure, Variant Selection, and Mechanical Properties of Laser-Welded Ti-4Al-2V Joints
by Yonghui Zhu, Lili Lu, Chenlu Zhang, Jun Yuan, Chao Fu and Lu Wang
Metals 2024, 14(4), 405; https://doi.org/10.3390/met14040405 (registering DOI) - 29 Mar 2024
Abstract
Laser welding of the near α-phase titanium alloy Ti-4Al-2V, used for complex components in the nuclear industry, has been rarely reported. In this study, butt weld joints made of Ti-4Al-2V alloy plates under different parameters, including the laser power, the welding speed, and [...] Read more.
Laser welding of the near α-phase titanium alloy Ti-4Al-2V, used for complex components in the nuclear industry, has been rarely reported. In this study, butt weld joints made of Ti-4Al-2V alloy plates under different parameters, including the laser power, the welding speed, and the defocus distance, were manufactured and analyzed. The results showed that adjusting the combination of 4.2 kW of laser power, a 20 mm/s welding speed, and a −2 mm defocus distance could achieve a penetration depth exceeding 6 mm. Porosity defects were prone to forming in the middle and bottom parts of the fusion zone, due to rapid cooling. The microstructure of the fusion zone was mainly needle-like α martensite, which precipitated in the form of specific clusters. The interior of a cluster was composed of three types of variants with <11−20>/60° phase interfaces to achieve the lower boundary’s energy. Affected by the microstructure and welding defects, the strength of the weld joint was basically similar under different welding conditions, namely about 720 MPa, slightly higher than that of the base metal, while the rupture elongation at breaking decreased by more than 50%. The micro-Vickers hardness of the weld joints was about 50–60 HV higher than that of the base metal, while the impact toughness was about 40 KJ, almost half that of the base metal. This research lays a solid foundation for the engineering application of laser welding of Ti-4Al-2V alloys. Full article
(This article belongs to the Special Issue Advanced Laser Welding Technologies for Metals and Alloys)
13 pages, 2604 KiB  
Article
Evaluation of Fluid Behaviors in a Pushbutton-Activated Microfluidic Device for User-Independent Flow Control
by Dong Hyun Han, Gihyun Lee, Untaek Oh, Yejin Choi and Je-Kyun Park
Micromachines 2024, 15(4), 465; https://doi.org/10.3390/mi15040465 (registering DOI) - 29 Mar 2024
Abstract
Although numerous studies have been conducted to realize ideal point-of-care testing (POCT), the development of a user-friendly and user-independent power-free microfluidic platform is still a challenge. Among various methods, the finger-actuation method shows a promising technique that provides a user-friendly and equipment-free way [...] Read more.
Although numerous studies have been conducted to realize ideal point-of-care testing (POCT), the development of a user-friendly and user-independent power-free microfluidic platform is still a challenge. Among various methods, the finger-actuation method shows a promising technique that provides a user-friendly and equipment-free way of delivering fluid in a designated manner. However, the design criteria and elaborate evaluation of the fluid behavior of a pushbutton-activated microfluidic device (PAMD) remain a critical bottleneck to be widely adopted in various applications. In this study, we have evaluated the fluid behavior of the PAMD based on various parameters, such as pressing velocity and depth assisted by a press machine. We have further developed a user-friendly and portable pressing block that reduces user variation in fluid behavior based on the evaluation. Full article
(This article belongs to the Special Issue Recent Advances of Microfluidics for Biomedical Applications)
14 pages, 5477 KiB  
Article
Whole-Genome Sequencing for Identifying Candidate Genes Related to the Special Phenotypes of the Taihu Dianzi Pigeon
by Rui Zhang, Chunyu Mu, Lingling Chang, Xinyue Shen, Zhu Bu, Mingjun Yang, Shengyong Fu, Qingping Tang, Peiyao Liu and Xiaoming Yang
Animals 2024, 14(7), 1047; https://doi.org/10.3390/ani14071047 (registering DOI) - 29 Mar 2024
Abstract
The Taihu Dianzi pigeon is a breed native to China, and its special piebalding, crest, and polydactyly phenotypes are the result of artificial and natural selection. Here, we analyzed the genetic differences among three kinds of pigeons with different phenotypes at the genomic [...] Read more.
The Taihu Dianzi pigeon is a breed native to China, and its special piebalding, crest, and polydactyly phenotypes are the result of artificial and natural selection. Here, we analyzed the genetic differences among three kinds of pigeons with different phenotypes at the genomic level. A selective sweep was conducted based on the fixation index (FST) and nucleotide diversity (π) ratio, and the results revealed that MC1R was related to the formation of the distinctive piebalding of the Taihu Dianzi pigeon. Combined with the results of genome-wide association studies, we identified candidate genes associated with the crest (SMYD and STOX2) and polydactyly (SLC52A3 and ANGPT4). The candidate genes identified in this study and their variants may be useful for understanding the genetic mechanism underlying the special phenotypes of the Taihu Dianzi pigeon. This study provides new insights into the genetic factors that may influence the formation of the special piebalding, crest, and polydactyly characteristics in pigeons. Full article
(This article belongs to the Section Birds)
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
21 pages, 4823 KiB  
Article
The “Restoration of the Restoration”: Investigation of a Complex Surface and Interface Pattern in the Roman Wall Paintings of Volsinii Novi (Bolsena, Central Italy)
by Claudia Pelosi, Filomena Di Stasio, Luca Lanteri, Martina Zuena, Marta Sardara and Armida Sodo
Coatings 2024, 14(4), 408; https://doi.org/10.3390/coatings14040408 (registering DOI) - 29 Mar 2024
Abstract
(1) Background: The present paper reports the results of the analyses performed on the wall paintings of Room E of the Domus delle Pitture (Room of the Paintings) in the Roman archaeological site of Volsinii Novi (Bolsena, Viterbo, Central Italy) aimed at supporting [...] Read more.
(1) Background: The present paper reports the results of the analyses performed on the wall paintings of Room E of the Domus delle Pitture (Room of the Paintings) in the Roman archaeological site of Volsinii Novi (Bolsena, Viterbo, Central Italy) aimed at supporting the recently concluded restoration. (2) Methods: ultraviolet fluorescence photography was conducted to evaluate the conservation state. Painting materials were investigated via portable X-ray fluorescence spectrometer (p-XRF), µ-Raman spectroscopy, cross-section analysis under polarizing microscope, scanning electron microscopy coupled with energy dispersive spectroscopy (SEM-EDS), and Fourier transform infrared (FTIR) spectroscopy. (3) Results: This analysis revealed the presence of traditional pigments used in Roman wall paintings, such as ochres, earths, and Egyptian blue. Additionally, it identified pigments from previous restoration containing titanium and chromium, along with other materials present in the surface and interface of the wall painting containing arsenic, copper, lead, and vanadium, where a bluish alteration was observed. In these samples, µ-Raman spectroscopy detected calcium oxalates but also copper arsenates, likely conichalcite and/or duftite. (4) Conclusions: This result is highly relevant from an analytical point of view and in relation to the restoration decisions. Addressing the irregular bluish stains on the wall paintings poses challenges in understanding, requiring a decision on whether to remove or retain them. Full article
(This article belongs to the Special Issue Surface and Interface Analysis of Cultural Heritage, 2nd Edition)
24 pages, 5507 KiB  
Article
Enhancing Wind Turbine Blade Preventive Maintenance Procedure through Computational Fluid Dynamics-Based Prediction of Wall Shear Stress
by Wasan Palasai, Chalermpol Plengsa-Ard and Mongkol Kaewbumrung
Sustainability 2024, 16(7), 2873; https://doi.org/10.3390/su16072873 (registering DOI) - 29 Mar 2024
Abstract
Wind turbine blades are essential parts of wind energy systems and are frequently exposed to harsh environmental elements, such as strong winds, turbulence, and corrosive atmospheric elements. Over time, these circumstances may result in serious harm to blades, such as delamination and erosion, [...] Read more.
Wind turbine blades are essential parts of wind energy systems and are frequently exposed to harsh environmental elements, such as strong winds, turbulence, and corrosive atmospheric elements. Over time, these circumstances may result in serious harm to blades, such as delamination and erosion, which may negatively affect the wind turbine’s functionality and durability. Accurate prediction of various types of damage is crucial to improve the toughness and lifespan of wind turbine blades and to maximize the overall effectiveness of wind energy systems. This article presents a novel computational fluid dynamics (CFDs)-based method for analyzing the distribution of wall shear stress on turbine blades, aimed at publicizing the yearly maintenance procedure. The investigation results from the CFDs, when compared with the current situation in a wind turbine farm in Thailand, confirmed that our wall shear stress modeling accurately predicted wind turbine damage. A maximum wall shear stress level higher than 5.00 Pa in the case of PA 90°, incoming air velocity 10.00 m/s, and 15 rpm was the main contribution to presenting the erosion and delamination from current drone inspection in wind turbine farms. In conclusion, these findings demonstrated the potential of using CFDs to predict wind turbine blade delamination and erosion, thereby significantly contributing to the development of specific and accurate yearly preventive maintenance. The proposed CFDs-based approach should serve as a sustainability tool for local human development, benefiting wind turbine engineers and operating technicians by providing them with a deeper understanding of the local flow conditions and wall shear stress distribution along wind turbine blades. This enables them to make informed decisions regarding blade design and maintenance. Full article
17 pages, 1688 KiB  
Article
Sea-Surface Small Target Detection Based on Improved Markov Transition Fields
by Ru Ye, Hongyan Xing and Xing Zhou
J. Mar. Sci. Eng. 2024, 12(4), 582; https://doi.org/10.3390/jmse12040582 (registering DOI) - 29 Mar 2024
Abstract
Addressing the limitations of manually extracting features from small maritime target signals, this paper explores Markov transition fields and convolutional neural networks, proposing a detection method for small targets based on an improved Markov transition field. Initially, the raw data undergo a Fourier [...] Read more.
Addressing the limitations of manually extracting features from small maritime target signals, this paper explores Markov transition fields and convolutional neural networks, proposing a detection method for small targets based on an improved Markov transition field. Initially, the raw data undergo a Fourier transform, feature fusion is performed on the series, and a spectrogram is generated using Markov transition fields to extract radar data features from both the time domain and frequency domain, providing a more comprehensive data representation for the detector. Then, the InceptionResnetV2 network is employed as a classifier, setting decision thresholds based on the softmax layer’s output, thus achieving controllable false alarms in the detection of small maritime targets. Additionally, transfer learning is introduced to address the issue of sample imbalance. The IPIX dataset is used for experimental verification. The experimental results show that the proposed detection method can deeply mine the differences between targets and the maritime clutter background, demonstrating superior detection performance. When the observation time is set to 1.024 s, the IMIRV2 detector performs best. Cross-validation with different data preprocessing methods and classification models reveals a significant advantage in the performance of the IMIRV2 detector, especially at low signal-to-noise ratios. Finally, a comparison with the performance of existing detectors indicates that the proposed method offers certain improvements. Full article
(This article belongs to the Section Ocean Engineering)
8 pages, 2878 KiB  
Case Report
A Case Report of Secondary Glaucoma in A Patient with HTLV-1: Discussion about the Management of Secondary Glaucoma Due to Recurrent Granulomatous Uveitis
by Kenta Ashikaga, Teruhiko Hamanaka, Mayumi Hosogai, Takao Tanaka, Shintaro Nakao and Toshinari Funaki
J. Clin. Transl. Ophthalmol. 2024, 2(2), 26-33; https://doi.org/10.3390/jcto2020003 (registering DOI) - 29 Mar 2024
Abstract
Purpose: to investigate secondary glaucoma resulting from uveitis in a patient infected with Human T-cell Leukemia Virus Type 1 (HTLV-1) pathologically and discuss the management of glaucoma with recurrent uveitis. Clinical course: An octogenarian woman diagnosed as a carrier of HTLV-1 experienced recurrent [...] Read more.
Purpose: to investigate secondary glaucoma resulting from uveitis in a patient infected with Human T-cell Leukemia Virus Type 1 (HTLV-1) pathologically and discuss the management of glaucoma with recurrent uveitis. Clinical course: An octogenarian woman diagnosed as a carrier of HTLV-1 experienced recurrent uveitis and a sudden rise in intraocular pressure (IOP) in both eyes. Due to the uncontrolled IOP and severely damaged visual field in her left eye, a combined procedure of trabeculectomy and DGIS (glaucoma drainage implant surgery, Baerveldt 350) was performed. The presence of HTLV-1 provirus was detected in the aqueous humor. Her trabeculectomy sample was processed for light microscopic observation. Following an irregular follow-up, she presented with a sudden decrease in vision and pain in her fellow eye, four years after the glaucoma surgeries. Her right eye exhibited a significant accumulation of mutton-fat-like keratic precipitates. Results: Clinical manifestations revealed the presence of granulomatous uveitis. The combined glaucoma surgery, along with continuous topical corticosteroid medication post-surgery in her left eye, effectively suppressed the high IOP spikes and the recurrence of uveitis for 4 years. The pathological examination of the outflow pathways showed a range of damages in Schlemm’s canal (SC), including SC endothelial loss, narrowing, and occlusion, as well as loss of trabecular meshwork (TM) cells and fused TM beams. Conclusion: Combined GDIS and trabeculectomy represents a promising approach for managing such refractory cases of secondary glaucoma. Continuous topical corticosteroid medication is strongly recommended to prevent irreversible changes in SC and TM associated with granulomatous uveitis. Full article
28 pages, 673 KiB  
Review
An Overview on the Big Players in Bone Tissue Engineering: Biomaterials, Scaffolds and Cells
by Maria Pia Ferraz
Int. J. Mol. Sci. 2024, 25(7), 3836; https://doi.org/10.3390/ijms25073836 (registering DOI) - 29 Mar 2024
Abstract
Presently, millions worldwide suffer from degenerative and inflammatory bone and joint issues, comprising roughly half of chronic ailments in those over 50, leading to prolonged discomfort and physical limitations. These conditions become more prevalent with age and lifestyle factors, escalating due to the [...] Read more.
Presently, millions worldwide suffer from degenerative and inflammatory bone and joint issues, comprising roughly half of chronic ailments in those over 50, leading to prolonged discomfort and physical limitations. These conditions become more prevalent with age and lifestyle factors, escalating due to the growing elderly populace. Addressing these challenges often entails surgical interventions utilizing implants or bone grafts, though these treatments may entail complications such as pain and tissue death at donor sites for grafts, along with immune rejection. To surmount these challenges, tissue engineering has emerged as a promising avenue for bone injury repair and reconstruction. It involves the use of different biomaterials and the development of three-dimensional porous matrices and scaffolds, alongside osteoprogenitor cells and growth factors to stimulate natural tissue regeneration. This review compiles methodologies that can be used to develop biomaterials that are important in bone tissue replacement and regeneration. Biomaterials for orthopedic implants, several scaffold types and production methods, as well as techniques to assess biomaterials’ suitability for human use—both in laboratory settings and within living organisms—are discussed. Even though researchers have had some success, there is still room for improvements in their processing techniques, especially the ones that make scaffolds mechanically stronger without weakening their biological characteristics. Bone tissue engineering is therefore a promising area due to the rise in bone-related injuries. Full article
16 pages, 9818 KiB  
Article
Dynamic Recrystallization Constitutive Model and Texture Evolution of Metastable β Titanium Alloy TB8 during Thermal Deformation
by Chuankun Zhou, Fang Cao, Zhirong Yang and Weifeng Rao
Materials 2024, 17(7), 1572; https://doi.org/10.3390/ma17071572 (registering DOI) - 29 Mar 2024
Abstract
The mechanical properties of metastable β-titanium alloys are highly susceptible during the thermal mechanical processing (TMP). In this process, the recrystallization process plays an important role in determining the microstructure and texture evolution. The implementation of dynamic recrystallization (DRX), a process for achieving [...] Read more.
The mechanical properties of metastable β-titanium alloys are highly susceptible during the thermal mechanical processing (TMP). In this process, the recrystallization process plays an important role in determining the microstructure and texture evolution. The implementation of dynamic recrystallization (DRX), a process for achieving β-grain refinement, is considered of great significance for the improvement of the properties of metastable β-titanium alloys and their industrial production. Along these lines, in this work, an isothermal compression test of TB8 titanium alloy was carried out by using a Gleeble-3500 thermal simulator. As a result, the rheological stress behavior was analyzed, the thermal processing map was accurately established based on the stress–strain curve, and the optimal processing interval was determined. The DRX kinetic and the DRX grain size models were developed, on the basis of which a new DRX intrinsic model was established to improve the material parameters. Therefore, the actual situation in the working process could be better predicted. The microstructural evolution of TB8 titanium alloy during thermal deformation was comprehensively investigated using the electron backscatter diffraction (EBSD) technique. The obtained results demonstrate a close correlation between the diversity of DRX mechanisms in TB8 alloy and the distribution of dislocation density. Four microstructural textures during thermal deformation were identified, in which the cube texture of (001) <010> and the R-Gorss Nd texture of (110) <110> dominate. Due to the random orientation of the dynamically recrystallized grains, the strength of the R-Gorss Nd texture of (110) <110> increases with the increase in the volume fraction of DRX. On the contrary, it was verified that the dynamic recrystallization behavior has a significant weakening impact on the cube texture of (001) <010>. Full article
(This article belongs to the Section Metals and Alloys)
11 pages, 1120 KiB  
Article
Calcium Chloride as a Novel Stabilizer for Foot-and-Mouth Disease Virus and Its Application in the Vaccine Formulation
by Jong Sook Jin, Gyeongmin Lee, Jae Young Kim, SooAh Lee, Jong-Hyeon Park, Sun Young Park and Young-Joon Ko
Vaccines 2024, 12(4), 367; https://doi.org/10.3390/vaccines12040367 (registering DOI) - 29 Mar 2024
Abstract
The thermal stability of the in-house-developed foot-and-mouth disease (FMD) type O and A viruses was evaluated, and the O Jincheon virus was found to exhibit the lowest thermal stability. To overcome this instability, we proposed a novel stabilizer, calcium chloride. The thermal stability [...] Read more.
The thermal stability of the in-house-developed foot-and-mouth disease (FMD) type O and A viruses was evaluated, and the O Jincheon virus was found to exhibit the lowest thermal stability. To overcome this instability, we proposed a novel stabilizer, calcium chloride. The thermal stability of FMDVs increased up to a CaCl2 concentration of 10 mM, and it had a decreasing trend at >30 mM. The O Jincheon virus showed a significant decrease in the amount of antigen over time at 4 °C. In contrast, the samples treated with CaCl2 showed stable preservation of the virus without significant antigen loss. After the CaCl2-formulated vaccine was administered twice to pigs, the virus neutralization titer reached approximately 1:1000, suggesting that the vaccine could protect pigs against the FMDV challenge. In summary, the O Jincheon virus is difficult to utilize as a vaccine given its low stability during storage after antigen production. However, following its treatment with CaCl2, it can be easily utilized as a vaccine. This study evaluated CaCl2 as a novel stabilizer in FMD vaccines and may contribute to the development of stable vaccine formulations, especially for inherently unstable FMDV strains. Full article
(This article belongs to the Special Issue Vaccine Development for Emerging and Zoonotic Diseases)
Show Figures

Figure 1

Full article ">Full article ">Full article ">
19 pages, 5627 KiB  
Article
Uncertainty-Guided Asymmetric Consistency Domain Adaptation for Histopathological Image Classification
by Chenglin Yu and Hailong Pei
Appl. Sci. 2024, 14(7), 2900; https://doi.org/10.3390/app14072900 (registering DOI) - 29 Mar 2024
Abstract
Deep learning has achieved remarkable progress in medical image analysis, but its effectiveness heavily relies on large-scale and well-annotated datasets. However, assembling a large-scale dataset of annotated histopathological images is challenging due to their unique characteristics, including various image sizes, multiple cancer types, [...] Read more.
Deep learning has achieved remarkable progress in medical image analysis, but its effectiveness heavily relies on large-scale and well-annotated datasets. However, assembling a large-scale dataset of annotated histopathological images is challenging due to their unique characteristics, including various image sizes, multiple cancer types, and staining variations. Moreover, strict data privacy in medicine severely restricts data sharing and poses significant challenges in acquiring large-scale and well-annotated histopathological images. To tackle these constraints, Transfer Learning (TL) provides a promising solution by exploiting knowledge from another domain. This study proposes the Uncertainty-guided asymmetric Consistency Domain Adaptation (UCDA), which does not require accessing the source data and is composed of two essential components, e.g., Uncertainty-guided Source-free Transfer Learning (USTL) and Asymmetric Consistency Learning (ACL). In detail, USTL facilitates a secure mapping of the source domain model’s feature space onto the target domain, eliminating the dependency on source domain data to protect data privacy. At the same time, the ACL module measures the symmetry and asymmetry between the source and target domains, bridging the information gap and preserving inter-domain differences among medical images. We comprehensively evaluate the effectiveness of UCDA on three widely recognized and publicly available datasets, namely NCTCRC-HE-100K, PCam, and LC25000. Impressively, our proposed method achieves remarkable performance on accuracy and F1-scores. Additionally, feature visualizations effectively demonstrate the exceptional generalizability and discriminative power of the learned representations. These compelling results underscore the significant potential of UCDA in driving the advancement of deep learning techniques within the realm of histopathological image analysis. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
19 pages, 1428 KiB  
Article
Improved Bootstrap Method Based on RBF Neural Network for Reliability Assessment
by Houxiang Wang, Haitao Liu and Songshi Shao
Appl. Sci. 2024, 14(7), 2901; https://doi.org/10.3390/app14072901 (registering DOI) - 29 Mar 2024
Abstract
The investigation of the reliability of long-life equipment is typically hindered by the lack of experimental data, which makes accurate assessments challenging. To address this problem, a bootstrap method based on the improved RBF (radial basis function) neural network is proposed. This method [...] Read more.
The investigation of the reliability of long-life equipment is typically hindered by the lack of experimental data, which makes accurate assessments challenging. To address this problem, a bootstrap method based on the improved RBF (radial basis function) neural network is proposed. This method utilizes the exponential function to modify the conventional empirical distribution function and fit right-tailed data. In addition, it employs the RBF radial basis neural network to obtain the distribution characteristics of the original samples and then constructs the neighborhood function to generate the input network. The expanded sample is used to estimate the scale and shape parameters of the Weibull distribution and obtain the estimated value of the MTBF (mean time between failures). The bias correction method is then used to obtain the interval estimate for the MTBF. Subsequently, a simulation experiment is conducted based on the failure data of a CNC (computer numerical control) machine tool to verify the effect of this method. The results show that the accuracy of the MTBF point estimation and interval estimation obtained using the proposed method is superior to those of the original and conventional bootstrap methods, which is of major significance to engineering applications. Full article
(This article belongs to the Special Issue Advances and Challenges in Reliability and Maintenance Engineering)
21 pages, 3695 KiB  
Article
Preclinical Development of Antisense Oligonucleotides to Rescue Aberrant Splicing Caused by an Ultrarare ABCA4 Variant in a Child with Early-Onset Stargardt Disease
by Nuria Suárez-Herrera, Catherina H. Z. Li, Nico Leijsten, Dyah W. Karjosukarso, Zelia Corradi, Femke Bukkems, Lonneke Duijkers, Frans P. M. Cremers, Carel B. Hoyng, Alejandro Garanto and Rob W. J. Collin
Cells 2024, 13(7), 601; https://doi.org/10.3390/cells13070601 (registering DOI) - 29 Mar 2024
Abstract
Precision medicine is rapidly gaining recognition in the field of (ultra)rare conditions, where only a few individuals in the world are affected. Clinical trial design for a small number of patients is extremely challenging, and for this reason, the development of N-of-1 strategies [...] Read more.
Precision medicine is rapidly gaining recognition in the field of (ultra)rare conditions, where only a few individuals in the world are affected. Clinical trial design for a small number of patients is extremely challenging, and for this reason, the development of N-of-1 strategies is explored to accelerate customized therapy design for rare cases. A strong candidate for this approach is Stargardt disease (STGD1), an autosomal recessive macular degeneration characterized by high genetic and phenotypic heterogeneity. STGD1 is caused by pathogenic variants in ABCA4, and amongst them, several deep-intronic variants alter the pre-mRNA splicing process, generally resulting in the insertion of pseudoexons (PEs) into the final transcript. In this study, we describe a 10-year-old girl harboring the unique deep-intronic ABCA4 variant c.6817-713A>G. Clinically, she presents with typical early-onset STGD1 with a high disease symmetry between her two eyes. Molecularly, we designed antisense oligonucleotides (AONs) to block the produced PE insertion. Splicing rescue was assessed in three different in vitro models: HEK293T cells, fibroblasts, and photoreceptor precursor cells, the last two being derived from the patient. Overall, our research is intended to serve as the basis for a personalized N-of-1 AON-based treatment to stop early vision loss in this patient. Full article
(This article belongs to the Special Issue Nucleic Acid Therapeutics (NATs): Advances and Perspectives)
12 pages, 4086 KiB  
Article
An Efficient Stereoselective Synthesis of cis-2,6-Disubstituted Tetrahydropyrans via Gold-Catalyzed Meyer–Schuster Rearrangement/Hydration/oxa-Michael Addition Sequence
by Nobuyoshi Morita, Daichi Yamashita, Yoshimitsu Hashimoto and Osamu Tamura
Catalysts 2024, 14(4), 228; https://doi.org/10.3390/catal14040228 (registering DOI) - 29 Mar 2024
Abstract
An efficient stereoselective synthesis of cis-2,6-disubstituted tetrahydropyrans 14ac has been achieved via gold-catalyzed Meyer–Schuster rearrangement/hydration/oxa-Michael addition sequence from bis-propargylic alcohols 13ac. The reaction of 13a proceeds via 2,6-disubstituted tetrahydropyran 14′a as an intermediate. Full article
(This article belongs to the Special Issue Advances in Transition Metal Catalysis)
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
21 pages, 1722 KiB  
Article
Inverse Analysis of Strata in Seepage Field Based on Regularization Method and Geostatistics Theory
by Fansheng Zhang, Lianglin Dong, Hongbo Wang, Ke Zhong, Peiyuan Zhang and Jinyan Jiang
Buildings 2024, 14(4), 946; https://doi.org/10.3390/buildings14040946 (registering DOI) - 29 Mar 2024
Abstract
During the construction of underground engineering, the prediction of groundwater distribution and rock body permeability is essential for evaluating the safety of the project and guiding subsequent design and construction. This article proposes an objective function that solves an underdetermined inverse analysis problem [...] Read more.
During the construction of underground engineering, the prediction of groundwater distribution and rock body permeability is essential for evaluating the safety of the project and guiding subsequent design and construction. This article proposes an objective function that solves an underdetermined inverse analysis problem based on the least-squares theory and regularization method and uses geostatistics theory and the variogram function to describe the spatial characteristics of the actual engineering system. It also establishes an optimization model of the analysis stratum seepage field and puts forward the method of using on-site test observation data to solve the stratum penetration coefficient. Relying on the foundation pit project of the Lingshanwei Station of Qingdao Metro, the on-site pumping and packer permeability test was conducted for different strata venues in the foundation pit, and the on-site water-head observation value was obtained. Physical detection of the influence area of foundation pit excavation confirms the correctness of the model from the region and verifies the accuracy of the model on the value through the on-site pumping test. Results show that the accuracy of the use of this objective function to solve the underdetermined inverse problem is above 85%, which proves the effectiveness of the method. The stratigraphic geological information obtained by the inverse analysis model provides an important basis for engineering design and security construction. Full article
(This article belongs to the Section Building Structures)
20 pages, 3591 KiB  
Article
A New Moment-Resisting Glulam Beam-End Connection Utilizing Mechanically Fastened Steel Rods—An Experimental Study
by Cory Hubbard and Osama (Sam) Salem
Appl. Mech. 2024, 5(2), 260-279; https://doi.org/10.3390/applmech5020016 (registering DOI) - 29 Mar 2024
Abstract
A new moment-resisting mass timber connection was designed based on the principles of force equilibrium in applied mechanics. The connection configuration utilizing two mechanically fastened threaded steel rods embedded into the end of a glulam beam section was experimentally investigated in this study. [...] Read more.
A new moment-resisting mass timber connection was designed based on the principles of force equilibrium in applied mechanics. The connection configuration utilizing two mechanically fastened threaded steel rods embedded into the end of a glulam beam section was experimentally investigated in this study. A gradually increasing transverse load was applied to the free end of a cantilevered beam, causing a bending moment on the beam-end connection until failure. Four different connection configurations were examined, each replicated twice to verify results. The beam connection parameters investigated were rod anchorage length (200 and 250 mm) and square washer size (38.1 and 50.8 mm). Test results show that increasing the washer size increased the connection bending strength by increments more significantly than those due to increasing the rod anchorage length. However, the connection configurations with the smaller-size washer, which failed mainly due to wood crushing under the washer, had higher ductility ratios than those with the larger-size washer, which failed due to steel rod yielding. In a real-life scenario, a structural element such as a glulam beam is usually loaded to approximately 50% to 70% of its design capacity, considering a reasonable margin of safety. The study estimates a maximum possible bending moment utilization factor for the strongest connection configuration that ranged between 34% and 48% compared to the maximum moment resistance of a supported glulam beam spanning an average length of 4.0 m to 6.0 m (a common span length in framed timber buildings) and has a cross-section size same as the one utilized in this study. This utilization factor is quite large for a timber connection, and thus, confirms a considerable moment-resisting capability of the new configuration developed in this study. Full article
21 pages, 3336 KiB  
Article
Green Hydrogen Production at the Gigawatt Scale in Portugal: A Technical and Economic Evaluation
by Rui P. Borges, Flávia Franco, Fátima N. Serralha and Isabel Cabrita
Energies 2024, 17(7), 1638; https://doi.org/10.3390/en17071638 (registering DOI) - 29 Mar 2024
Abstract
The European Union has committed to achieving carbon neutrality by 2050 and green hydrogen has been chosen as a priority vector for reaching that goal. Accordingly, Portugal has drafted a National Hydrogen Strategy laying out the various steps for the development of a [...] Read more.
The European Union has committed to achieving carbon neutrality by 2050 and green hydrogen has been chosen as a priority vector for reaching that goal. Accordingly, Portugal has drafted a National Hydrogen Strategy laying out the various steps for the development of a green hydrogen economy. One element of this strategy is the development of a gigawatt-scale hydrogen production facility powered by dedicated renewable electricity sources. This work presents an analysis of the technical and economic feasibility of a facility consisting of a gigawatt-scale polymer electrolyte membrane electrolyser powered by solar photovoltaic and wind electricity, using the energy analysis model EnergyPLAN. Different capacities and modes of operation of the electrolyser are considered, including the complementary use of grid electricity as well as different combinations of renewable power, resulting in a total of 72 different configurations. An economic analysis is conducted addressing the related annualised capital expenditures, maintenance, and variable costs, to allow for the determination of the levelised cost of hydrogen for the different configurations. This analysis shows the conditions required for maximising annual hydrogen production at the lowest levelised cost of hydrogen. The best options consist of an electrolyser powered by a combination of solar photovoltaic and wind, with limited exchanges with the electricity grid, and a levelised cost of hydrogen in the range 3.13–3.48 EUR/kg. Full article
(This article belongs to the Special Issue Hydrogen in the Energy Transition: From Production to End-Use)
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">Full article ">
15 pages, 1356 KiB  
Article
A Novel Two-Stage 3D-Printed Halbach Array-Based Device for Magneto-Mechanical Applications
by Antonios Makridis, Nikolaos Maniotis, Dimitrios Papadopoulos, Pavlos Kyriazopoulos and Makis Angelakeris
Magnetochemistry 2024, 10(4), 21; https://doi.org/10.3390/magnetochemistry10040021 (registering DOI) - 29 Mar 2024
Abstract
This research unveils a versatile Halbach array magnetic device with promising biomedical applications, offering innovative solutions for targeted therapy and disease management in evolving biomedical engineering. This paper explores the potential of a novel Halbach array-based device for harnessing magneto-mechanical phenomena in biomedical [...] Read more.
This research unveils a versatile Halbach array magnetic device with promising biomedical applications, offering innovative solutions for targeted therapy and disease management in evolving biomedical engineering. This paper explores the potential of a novel Halbach array-based device for harnessing magneto-mechanical phenomena in biomedical applications. The study employs computational modeling using COMSOL Multiphysics to define the device’s magnetic properties and validate its operation within the theoretical prediction. The research catalogs the device’s operational modes and assesses crucial parameters related to magneto-mechanical biomedical modalities, including magnetic field strength, gradient, and force. Experimental validation of numerical findings through magnetic field measurements confirms the device’s multifaceted potential, particularly in targeted drug delivery and tissue engineering applications. Finally, the adaptability of the magnetic arrangements for various scenarios is also highlighted. This investigation provides valuable insights into integrating magneto-mechanical principles into biomedical engineering. It paves the way for further research and innovative approaches in theranostics, positioning the presented apparatus as a promising tool with untapped potential for future exploration and discovery in the evolving biomedical field. Full article
(This article belongs to the Special Issue Magnetism: Energy, Recycling, Novel Materials)
15 pages, 2891 KiB  
Article
Longitudinal Sequencing and Variant Detection of SARS-CoV-2 across Southern California Wastewater
by Jason A. Rothman, Andrew Saghir, Amity G. Zimmer-Faust, Kylie Langlois, Kayla Raygoza, Joshua A. Steele, John F. Griffith and Katrine L. Whiteson
Appl. Microbiol. 2024, 4(2), 635-649; https://doi.org/10.3390/applmicrobiol4020044 (registering DOI) - 29 Mar 2024
Abstract
Wastewater-based epidemiology (WBE) is useful for detecting pathogen prevalence and may serve to effectively monitor diseases across broad scales. WBE has been used throughout the COVID-19 pandemic to track disease burden through quantifying SARS-CoV-2 RNA present in wastewater. Aside from case load estimation, [...] Read more.
Wastewater-based epidemiology (WBE) is useful for detecting pathogen prevalence and may serve to effectively monitor diseases across broad scales. WBE has been used throughout the COVID-19 pandemic to track disease burden through quantifying SARS-CoV-2 RNA present in wastewater. Aside from case load estimation, WBE is being used to assay viral genomic diversity and emerging potential SARS-CoV-2 variants. Here, we present a study in which we sequenced RNA extracted from sewage influent obtained from eight wastewater treatment plants representing 16 million people in Southern California from April 2020 to August 2021. We sequenced SARS-CoV-2 with two methods: Illumina Respiratory Virus-Enriched metatranscriptomic sequencing (N = 269), and QIAseq SARS-CoV-2-tiled amplicon sequencing (N = 95). We classified SARS-CoV-2 reads into lineages and sublineages that approximated named variants and identified single nucleotide variants (SNVs), of which many are putatively novel SNVs and SNVs of unknown potential function and prevalence. Through our retrospective study, we also show that several SARS-CoV-2 sublineages were detected in wastewater before clinical detection, which may assist in the prediction of future variants of concern. Lastly, we show that sublineage diversity was similar across Southern California and that diversity changed over time, indicating that WBE is effective across megaregions. As the COVID-19 pandemic moves to new phases, and SARS-CoV-2 variants emerge, monitoring wastewater is important to understand local- and population-level dynamics of the virus. These results will aid in our ability to monitor the evolutionary potential of SARS-CoV-2 and help understand circulating SNVs to further combat COVID-19. Full article
Show Figures

Figure 1

Full article ">Full article ">Full article ">Full article ">Full article ">

Open Access Journals

Browse by Indexing Browse by Subject Selected Journals
Back to TopTop
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news
news